| 缺硫对莱茵衣藻叶绿素荧光参数和产H2速率的影响 |
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| 引用本文： | 贺立静.缺硫对莱茵衣藻叶绿素荧光参数和产H2速率的影响[J].安徽农业科学,2007,35(8):2222-2224. |
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| 作者姓名： | 贺立静 |
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| 作者单位： | 暨南大学水生生物研究所,广东广州,510632 |
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| 基金项目： | 广东省科技攻关项目（2004833401002）;国家“863”计划项目（2002AA515030）. |
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| 摘 要： | 采用TAP及TAP-S培养基培养莱茵衣藻(Chlamydomonas reinhardtii D.),测定了该藻叶绿素荧光参数及产氢气能力.结果表明:莱茵衣藻在TAP培养基内生长良好,有微量氢气产生,最高产氢速率只有1.4×10-4 ml/(mgChl·h).在TAP-S培养基内,莱茵衣藻的荧光参数Fv/Fo、ΦPSⅡ、qp分别在40～48 h下降到初始值的50%,说明缺硫对藻光合作用的影响首先发生在天线色素到PSⅡ反应中心的传能过程以及光合作用暗反应所需的酶.TAP-S培养基内藻最高产氢速率达0.22ml/(mgChl·h),缺硫可以显著提高莱茵衣藻氢气产生的速率.
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| 关 键 词： | 缺硫 莱茵衣藻 叶绿素荧光 氢气 |
| 文章编号： | 0517-6611（2007）08-02222-03 |
| 修稿时间： | 2006-12-11 |
EEffects of Sulfur Deficiency on Chlorophyll Fluorescence and Hydrogen Production in Chlamydomonas reinhardtii |
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| HE Li-jing.EEffects of Sulfur Deficiency on Chlorophyll Fluorescence and Hydrogen Production in Chlamydomonas reinhardtii[J].Journal of Anhui Agricultural Sciences,2007,35(8):2222-2224. |
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| Authors: | HE Li-jing |
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| Institution: | Institute of Hydrobiology, Jinan University, Guangzhou, Guangdong 510632 |
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| Abstract: | Chlorophyll fluorescence and hydrogen production was measured in a unicellular green alga(Chlamydomonas reinhardti).In TAP medium C.reinhardti slowly produced hydrogen.The maximum rate of H2 evolution was 1.4×10-4 ml/(mgChl·h).In TAP-S medium chlorophyll fluorescence parameters Fv/Fo,ΦPSII,qP decreased 50 % of their initial values at 40~48 h,respectively.This result showed that sulfur deficiency inhibited the activity of enzymes involving dark reaction and energy transport process from antenna chlorophyll to react center of PS.The maximum rate of H2 produced by C.reinhardti in sulfur-deprived medium was 0.22 ml/(mgChl·h).The rate of H2 evolution was markedly improved in TAP-S culture medium. |
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| Keywords: | Sulfur deprivation Chlamydomonas reinhardtii Chlorophyll fluorescence Hydrogen |
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