| 周龄·初始注射时间对小鼠超数排卵的影响 |
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| 引用本文： | 巩雪洁,臧敦安,许文豪.周龄·初始注射时间对小鼠超数排卵的影响[J].安徽农业科学,2018,46(15):72-74. |
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| 作者姓名： | 巩雪洁 臧敦安 许文豪 |
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| 作者单位： | 中南民族大学生命科学学院医学生物研究所,湖北武汉,430074;中南民族大学生命科学学院医学生物研究所,湖北武汉,430074;中南民族大学生命科学学院医学生物研究所,湖北武汉,430074 |
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| 摘 要： | 目的]探讨不同周龄、不同初始注射时间对小鼠超数排卵效果的影响。方法]用10 IU PMSG+10 IU HCG间隔48 h处理C57BL/6J小鼠,收集并统计合子数。结果]4周龄超排效果优于于8周龄,可达26.86枚∕只;4周龄C57BL/6J小鼠PMSG和HCG注射时间为12:00—13:00,超排后收集合子数可达30枚∕只以上,而且体外培养的发育状况良好。结论]4周龄C57BL/6J小鼠适宜超数排卵;PMSG和HCG注射时间为12:00—13:00,超排效果相对理想。
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| 关 键 词： | 小鼠 超排卵 激素 周龄 初始注射时间 |
The Influence of Week-age and Initial Injection Time on Superovulation in Mice |
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| GONG Xue-jie,ZANG Dun-an,XU Wen-hao.The Influence of Week-age and Initial Injection Time on Superovulation in Mice[J].Journal of Anhui Agricultural Sciences,2018,46(15):72-74. |
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| Authors: | GONG Xue-jie ZANG Dun-an XU Wen-hao |
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| Abstract: | Objective]To discuss the influence of week-age and initial injection time on superovulation in mice.Method]The C57BL/6J mice were treated with 10 IU PMSG+10 IU HCG interval 48h,and the number of zygotes was collected and counted.Result]The effect of the four week age superovulation was better than that of eight weeks,and it could reach 26.86.The time of PMSG and HCG injection of C57BL/6J mice at 4 weeks old was 12-13 at noon,and the number of zygotes collected was up to 30 and in vitro culture of the fertilized ovum was in good condi-tion.Conclusion]The 4-week old C57BL/6J mice are suitable for superovulation.When the injection time of PMSG and HCG is 12-13 at noon, the superovulation effect is relatively ideal. |
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