| 不同培养条件对枸杞组培苗玻璃化的影响 |
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| 引用本文： | 罗青,张曦燕,李晓莺,曹有龙.不同培养条件对枸杞组培苗玻璃化的影响[J].安徽农业科学,2008,36(22). |
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| 作者姓名： | 罗青 张曦燕 李晓莺 曹有龙 |
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| 作者单位： | 宁夏农林科学院枸杞工程技术研究中心,宁夏银川,750002 |
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| 基金项目： | 国家成果转化基金，国家自然科学基金 |
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| 摘 要： | 目的]探讨不同培养条件对枸杞组培苗玻璃化的影响。方法]以"宁杞3号"的幼嫩茎段为外植体,以MS为基本培养基,研究6-BA浓度、蔗糖浓度、琼脂浓度、培养温度、光照时间对枸杞组培苗玻璃化现象的影响。结果]当6-BA浓度为0.2 mg/L、蔗糖浓度为3%、琼脂浓度为6.5 g/L、培养温度25℃、光照时间12 h/d(日光灯,光照强度2000 lx)时,枸杞组培苗繁殖系数最高,玻璃化率较低。结论]综合调控枸杞组织培养条件,可以减少玻璃化现象的发生。
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| 关 键 词： | 枸杞 组织培养 玻璃化 影响因素 |
Effects of Different Culture Conditions on the Vitrification of Tissue Culture Seedlings of Lycium barbarum L. |
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| LUO Qing et al.Effects of Different Culture Conditions on the Vitrification of Tissue Culture Seedlings of Lycium barbarum L.[J].Journal of Anhui Agricultural Sciences,2008,36(22). |
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| Authors: | LUO Qing |
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| Abstract: | Objective] The study aimed to discuss the effects of different culture conditions on the vitrification of Lycium barbarum L. seedlings.Method] With the tender stems of Ningqi 3 as the explants and MS as basic medium,the effects of 6-BA concn.,cane sugar concn.,agar concn.,culture temperature and illumination time on the vitrification of tissue culture seedlings of L.barbarum were studied.Result]When 6BA was 0.2 mg/L,sucrose was 3%,agar was 6.5 g/L,culture temperature is 25 ℃ and illumination time is 12 h/d resp.,the tissue culture seedlings of L.barbarum had the highest bud multiplication coefficient and the lowest vitrification rate.Conclusion] Regulating the tissue culture conditions of L.barbarum comprehensively could reduce the occurence of the vitrification phenomena. |
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| Keywords: | Lycium barbarum L Tissue culture Vitrification Influencing factor |
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