缩胆囊素主动免疫对猪生产性能的影响

[目的]利用分子生物学技术扩增猪CCK基因活性片段,检验主动免疫CCK融合蛋白对肉猪生长是否有促进作用。[方法]采用RT-PCR技术成功扩增猪缩胆囊素基因C-端活性片段,并在大肠杆菌中进行表达。[结果]主动免疫0.5和1.5mg/mlCCK疫苗处理组肉猪,试验期肉猪平均日增重较空白对照组分别提高5.81%和7.36%（P〈0.05）,采食量分别提高4.04%和2.53%。利用SDS-PAGE可获得相对分子质量45000的融合蛋白。融合蛋白主要以可溶形式表达。Wesrtern Blot证实融合蛋白与CCK-8阳性血清具有良好的免疫反应性。[结论]主动免疫CCK对肉猪生长具有一定促进作用。

Effect of Active Immunization against Cholecystokinin on the Growth Performance of Pigs

[Objective]The active fragment of cholecystokinin gene of pigs was amplified by using molecular biological technology and it was tested that whether active immunization against cholecystokinin fusion protein can promote the growth of meat pigs.[Method]C-terminal fragment of cholecystokinin gene of pigs were successfully amplified by RT-PCR and expressed in E.coli BL-21.[Result]The average daily gain of meat pigs in the treatment group of 0.5 and 1.5 mg/ml CCK vaccine by active immunization in the test period increased by 5.81% and 7.36% than blank control group(P < 0.05) and the feed intake increased by 4.04% and 2.53% respectively.Using SDS-PAGE,the fusion protein was obtained with molecular weight of 45 000.Most fusion proteins were expressed in soluble form.Western blot showed that the expression products had a good immunoreactivity to the positive serum of CCK-8.[Conclusion]Active immunization against choleystokinin may promote the growth of meat pigs.