芸豆不同发芽阶段生物类黄酮对DPPH自由基的清除效率研究

目的]探讨芸豆不同发芽阶段生物类黄酮对DPPH自由基的清除效率。方法]芸豆种子经保温保湿催芽,分别于发芽1、3、5、7、9 d取出烘干,经乙醇提取得不同发芽阶段生物类黄酮提取液,并进行DPPH自由基清除率的测定。结果]芸豆种子发芽后,黄酮类化合物含量下降,且发芽时间越长,黄酮类化合物含量下降越多;随着芸豆发芽时间的延长,黄酮提取液对DPPH自由基的清除率总体呈下降趋势,同一发芽阶段,随着原液稀释程度的提高,对DPPH自由基的清除率总体也表现出下降趋势;芸豆不同发芽阶段黄酮对DPPH自由基的清除率均大于相同浓度Vc溶液。结论]芸豆黄酮具有较强的清除自由基能力。

Study of Scavenging Activities on DPPH Free Radical by Bioflavonoid of Kidney Beans at Different Germination Periods

Objective] To explore the scavenging activities on DPPH free radical of bioflavonoid of kidney beans under different germination periods.Methods] After temperature-keeping and humidity-keeping germinating of kidney beans seeds,1,3,5,7 and 9 day-old germinating seeds were taken and dried then extracted with ethanol for preparing bioflavonoid extracts under different germination periods whose DPPH scavenging activities were also investigated.Results] The bioflavonoid content of kidney beans decreased after germination,and the lesser of the content,the longer of the germination time;the scavenging activities on DPPH free radical tended to decrease with the increase of germination time,which also decreased with the increasing dilution degrees under the same germination period;DPPH scavenging rates of bioflavonoid extracts made under different germination periods were far bigger than the vitamin C of the same concentration.Conclusion] The bioflavonoid of kidney beans had stronger capability to scavenge the free radical.