| 用DNS光度法测定还原糖的条件研究 |
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| 引用本文： | 杨贵明,蒋爱华,薛秋生.用DNS光度法测定还原糖的条件研究[J].安徽农业科学,2006,34(14):3258-3258,3264. |
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| 作者姓名： | 杨贵明 蒋爱华 薛秋生 |
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| 作者单位： | 承德医学院蚕业研究所,河北,承德,067000;承德医学院蚕业研究所,河北,承德,067000;承德医学院蚕业研究所,河北,承德,067000 |
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| 摘 要： | 研究了用DNS光度法测定还原糖的影响因素。结果表明:DNS试剂的适宜用量为2.0 ml;样品及标准溶液测定时,显色液体积必须一致;沸水浴显色时间不低于2 min,显色定容30 min后于520 nm处测定有色溶液的吸光度。
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| 关 键 词： | 还原糖 DNS 光度法 测定条件 |
| 文章编号： | 0517-6611（2006）14-3258-01 |
| 收稿时间： | 2006-04-11 |
| 修稿时间： | 2006-04-11 |
Study on the Determination Factor of Reduced Sugar with DNS Spectrophotometry |
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| YANG Gui- ruing, et al.Study on the Determination Factor of Reduced Sugar with DNS Spectrophotometry[J].Journal of Anhui Agricultural Sciences,2006,34(14):3258-3258,3264. |
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| Authors: | YANG Gui- ruing |
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| Institution: | Institute of Sericuhure, Chengde Medical College, Chengde, Hebci 067000 |
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| Abstract: | The results of determination of reducing sugar with DNS spectrophotometry showed that the proper volume of DNS sample was 2.0 ml.The solution volume of samples and standard substances must be kept the same when reacted.Colour reaction in the condition of boiling water bath was kept for at least 2 minutes.Absorbance of the coloured solution was determined at the wavelength of 520 nm for 30 minutes for the volume-making. |
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| Keywords: | Reducing sugar DNS Spectrophotometry Determination factor |
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