| 2株H9N2亚型禽流感病毒全基因组序列测定及遗传性分析 |
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| 引用本文： | 沈佳,章振华,姜北宇,李林,景小冬,张建伟.2株H9N2亚型禽流感病毒全基因组序列测定及遗传性分析[J].安徽农业科学,2011(9):5241-5244. |
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| 作者姓名： | 沈佳 章振华 姜北宇 李林 景小冬 张建伟 |
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| 作者单位： | 北京市农林科学院畜牧兽医研究所; |
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| 基金项目： | 国家973资助项目子课题(2005CB523001) |
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| 摘 要： | 目的]探讨2株H9N2分离毒株与其他参考毒株的全基因组遗传变异关系。方法]采用RT-PCR技术扩增了2株H9N2亚型禽流感病毒分离株8个基因片段的全基因序列,并对所得序列与6个参考毒株进行了同源性比较及遗传进化树分析。结果]2个毒株与6个参考毒株全基因组同源性在91.1%～95.4%,PG08与C/BJ/1/94的全基因序列的核苷酸同源性最高为91.3%;而ZD06与D/HK/Y280/97最高为92.3%。2个分离株的HA基因裂解位点均为PARSSR/GLF,均为H9N2低致病力禽流感病毒。结论]ZD06株与C/Pak/2/99的亲缘关系最近,属欧亚种系;PG08株与其他毒株亲缘关系稍远,它可能是不同亚支基因重排的产物。
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| 关 键 词： | 禽流感病毒 H9N2 全基因组 序列分析 |
Complete Genome Sequencing and Genetic Variation Analysis of Two H9N2 Subtype Avian Influenza Virus Strains |
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| SHEN Jia et al.Complete Genome Sequencing and Genetic Variation Analysis of Two H9N2 Subtype Avian Influenza Virus Strains[J].Journal of Anhui Agricultural Sciences,2011(9):5241-5244. |
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| Authors: | SHEN Jia |
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| Institution: | SHEN Jia et al(Institute of Animal Science,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097) |
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| Abstract: | Objective] The study aimed to investigate the genetic variation characters of entire sequences between two H9N2 subtype avian influenza virus strains and other reference strains.Method] The entire sequences of 8 genes were obtained by using RT-PCR,and these sequences were analyzed with that of six H9N2 subtype avian influenza isolates in homology comparison and genetic evolution relation.Result] The results showed that the nucleotide sequence of entire gene of the strain shared 91.1%-95.4% homology with ... |
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| Keywords: | Avian influenza virus H9N2 subtype Complete genome Sequence analysis |
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