紫花芸豆胰蛋白酶抑制剂部分性质研究

目的]研究紫花芸豆（Phaseolus vulgaris）胰蛋白酶抑制剂（PVTI）的部分性质。方法]探索了不同温度、不同还原剂和变性剂处理对PVTI抑制活性和蛋白构象的影响。结果]PVTI是一种对热稳定蛋白;部分二硫键对活性中心有重要贡献;经尿素处理,PVTI的荧光光谱没有明显变化,但残留了较少的抑制活性,推测亚基的解离影响了抑制活性而每个亚基的内部结构未受到影响;经盐酸胍处理,Trp残基完全暴露于极性环境中,整个蛋白质分子处于一种松散伸展的状态,PVTI活性全部丧失。结论]该研究可为PVTI的功能研究及其进一步应用奠定基础。

Partial Characterization of Trypsin Inhibitor from Phaseolus vulgaris(PVTI)

Objective] The research aimed to study the partial characterization of PVTI.Method] The inhibitor activity and molecular conformation of PVTI under conditions of different temperature,reducer and denaturant were studied.Method] PVTI was a protein of thermal stability.Some disulfide bond was an important necessary for activity.Fluorescence spectrum of PVTI showed unconspicuous changes at 8 mol/L Urea,but it affected active center.Probably It affected inhibitor activity to undo submits,but the inner struct...