| 暗紫红毛菜ITS序列的测定与分析 |
| |
| 引用本文： | 李峰,冯佳,谢树莲.暗紫红毛菜ITS序列的测定与分析[J].安徽农业科学,2011(9):5116-5117. |
| |
| 作者姓名： | 李峰 冯佳 谢树莲 |
| |
| 作者单位： | 山西大学生命科学学院; |
| |
| 基金项目： | 国家自然科学基金项目(30970187) |
| |
| 摘 要： | 目的]对暗紫红毛菜rDNA内转录间隔区（ITS区）进行序列测定,为其系统发育研究提供新资料。方法]以产于山西娘子关泉的一株暗紫红毛菜为材料,提取DNA,设计引物,进行PCR扩增,进而测定其ITS区基因序列。结果]暗紫红毛菜与同科的少精紫菜ITS区同源性为75%,与条斑紫菜ITS区同源性为79%。结论]ITS区序列进化速率相对较快,种类和地理分布的差异是其序列差异产生的原因。
|
| 关 键 词： | 暗紫红毛菜 ITS1-5.8S rDNA-ITS2 序列测定 |
Sequencing and Analysis of ITS Sequences of Bangia atropurpurea |
| |
| LI Feng et al.Sequencing and Analysis of ITS Sequences of Bangia atropurpurea[J].Journal of Anhui Agricultural Sciences,2011(9):5116-5117. |
| |
| Authors: | LI Feng |
| |
| Institution: | LI Feng et al(School of Life Science,Shanxi University,Taiyuan,Shanxi 030006) |
| |
| Abstract: | Objective] To providing new data for phylogenesis of Bangia atropurpurea by the sequencing of internal transcribed spacer(ITS region) of rDNA.Method] B.atropurpurea was collected from Niangziguan Spring in Shanxi,the DNA was extracted and the primers were designed for PCR amplification so as to obtain ITS gene sequence.Result] The homology of ITS region between B.atropurpurea and Porphyra oligospermatangia was 75%,which was 79% between B.atropurpurea and P.yezoensis.Conclusion] Compared with other genes... |
| |
| Keywords: | Bangia atropurpurea ITS1-5 8SrDNA-ITS2 Sequence |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
