黄芪多糖单糖组分的气相色谱分析

目的]研究黄芪多糖的单糖组成。方法]以2 mol/L硫酸水解黄芪多糖,然后采用吡啶溶解干燥的水解产物,结合三甲基硅烷进行衍生,最后用毛细管气相色谱法测定黄芪多糖的单糖组成。结果]鉴定出黄芪多糖中含有阿拉伯糖、果糖、葡萄糖和甘露糖,并测定计算出阿拉伯糖-果糖-葡萄糖-甘露糖的摩尔构成比例为1∶10.309∶24.667∶0.462;另外还鉴定出了3种未知物,他们的相对保留时间分别为10.627、14.783和18.249 min。结论]该方法简便灵敏,可以用于黄芪多糖中单糖的组分分析。

Monosaccharide Composition in RADIX ASTRAGALI Polysaccharides by Gas Chromatography

Objective] To research on the composition of monosaccharide from RADIX ASTRAGALI polysaccharides.Method] RADIX ASTRAGALI polysaccharides sample was hydrolyzed with 2 mol/L sulfuric acid.Then,the dried hydrolysate was dissolved in pyridine,and derivatization was conducted by trimethylsilyl ether.Finally,the composition of monosaccharide from RADIX ASTRAGALI polysaccharides was detected by Capillary Gas Chromatography.Result] Arabinose,fructose,glucose,mannose were identified from the RADIX ASTRAGALI polysaccharides.And their molar ratio calculated according to the equation was 1∶ 10.309∶ 24.667∶ 0.462.Besides,three unknown matters were detected,with the retention time being 10.627,14.783 and 18.249 min.Conclusion] This method was simple and sensitive,and could be used for the component analysis of monosaccharide in RADIX ASTRAGALI polysaccharide.