鸡传染性法氏囊病高免血清的制备与检验

目的]探索一种新的制备鸡传染性法氏囊病高免血清的方法。方法]采用IBDV灭活苗对27日龄SPF鸡作基础免疫,活疫苗作3次加强免疫,四免后21 d采血制备高免血清。结果]试验结果表明随着免疫次数的增加,试验鸡IBD中和抗体滴度逐步增加,一免和二免后中和抗体分别为12 log2和13 log2,三免和四免后中和抗体达到最高值15 log2。结论]采用该方法免疫SPF鸡,有效保护了法氏囊不受损伤,3次免疫即可获得高效价的IBDV抗血清。

Preparation and Test of the Hyperimmune Serum on Chicken Infectious Bural Disease

Objective] To explore a new method for preparing the hyperimmune serum on chicken infectious bural disease.Method] 27 day-old SPF chickens were immunized with IBDV inactivated vaccine as the basic immunization,and three booster immunization with IBDV live vaccine were operated,the hyper immunized serum was prepared 21 days after the fourth immunization.Result] The titre of neutralizing antibody increased with immunization times,the values of neutralizing antibody titer were 12 log2 and 13 log2 after the first and second immunization respectively,and reached the highest value of 15 log2 after the third and fourth immunization.Conclusion] The bursa fabricius of vaccinated SPF chickens can be protected by this method,and the high-titer serum against IBDV can be prepared though three immunizations.