水回流法提取河湟红花籽粕中5-羟色胺衍生物的工艺研究

目的]优选水回流法提取河湟红花籽粕5-羟色胺衍生物的最佳工艺条件。方法]以5-羟色胺衍生物含量为指标,采用正交试验法对影响水回流法提取河湟红花粉粕中5-羟色胺衍生物的提取温度、提取时间、提取次数和料液比4个因素进行考察,优选出最佳提取方案。结果]影响蒸馏水回流法提取河湟红花籽粕中5-羟色胺衍生物含量的因素依次为提取温度提取时间提取次数料液比;提取温度对5-羟色胺衍生物提取率具有明显影响;最佳提取方案为:提取温度80℃,提取时间1.5 h,提取次数3次,料液比1∶20(g/ml);在此条件下提取的5-羟色胺衍生物含量为0.66%。结论]优选出了最佳提取工艺,为河湟红花籽粕的开发利用提供了理论依据。

Study on the Extraction Technology of Serotonin Derivatives from Seed Meal of Hehuang Carthamus tinctorius L.by Water Reflux Method

Objective] To optimize the extraction technology of serotonin derivatives from seed meal of Hehuang Carthamus tinctorius L.by water reflux method.Method] Taking the content of serotonin derivatives as index,the extraction technology of serotonin derivatives was optimized by means of orthogonal test with extraction temperature,extraction time,extraction times and solid-liquid ratio as experimental factors.Result] The extraction of serotonin derivatives from seed meal of Hehuang C.tinctorius was impacted mostly by extraction temperature,followed by extraction time,extraction times and solid-liquid ratio.The optimal technology was extraction temperature of 80 ℃,extraction time of 1.5 h,extraction times of 3 and solid-liquid ratio of 1∶ 20 g/ml,with which the content of f serotonin derivatives was 0.70%.Conclusion] This optimal extraction technology could provide basis for the development and production of Hehuang C.tinctorius seed meal.