3株泰山虫草无性型的分子生物学研究 |
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引用本文: | 赵品苍,曲志才,王波,苏延友. 3株泰山虫草无性型的分子生物学研究[J]. 安徽农业科学, 2012, 40(9): 5143-5144,5147 |
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作者姓名: | 赵品苍 曲志才 王波 苏延友 |
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作者单位: | 曲阜师范大学生命科学学院,山东曲阜,273165;泰山医学院药物科学学院,山东泰安,271000 |
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基金项目: | 山东省自然科学基金;泰山地区虫草资源的分子系统学分析(ZR2009DM038) |
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摘 要: | [目的]研究3株泰山虫草无性型的分子生物学特性。[方法]以野生泰山虫草子实体分离出的3个菌株为材料,通过对rDNAITS区的扩增、克隆和测序来鉴定虫草的无性型。[结果]3个菌株不是同1种,COA1为蛹草拟青霉,COA2为球孢白僵菌,COA3为细脚棒束孢,由此推断泰山虫草只是以地理位置得名。[结论]研究了3株泰山虫草无性型的分子生物学特性。
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关 键 词: | 泰山虫草 无性型 rDNA ITS 蛹草拟青霉 球孢白僵菌 细脚棒束孢 |
Molecular Research on the Anamorph of Three Strains of Cordyceps taishanensis |
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Affiliation: | ZHAO Pin-cang et al(College of Life Science,Qufu Normal University,Qufu,Shandong 273165) |
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Abstract: | [Objective] To identify the anamorph of Cordyceps taishanensis.[Method] The rDNA ITS fragment was amplified,cloned and sequenced using total DNA extracted from three strains of COA1,COA2 and COA3.[Result] COA1 was Paecilomyces militaris,COA2 was Beauveria bassina and COA3 was Isaria tenuipes.The findings indicated that Cordyceps taishanensis was named based on the geographical position.[Conclusion] Molecular biological characteristics of three tested strains are studied. |
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Keywords: | Cordyceps taishanensis Anamorph rDNA ITS Paecilomyces militaris Beauveria bassina Isaria tenuipes |
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