采用叶绿素成像技术测定细叶榕(Ficus microcarpa L.)叶片对聚乙二醇模拟的水分胁迫的响应

目的]采用叶绿素成像技术测定细叶榕(Ficus microcarpa L.)叶片对聚乙二醇模拟的水分胁迫的响应。方法]人工模拟水分胁迫下检测细叶榕、垂叶榕和极端耐旱的欧洲夹竹桃离体叶片,并用叶绿素荧光成像技术同时提取单片叶多个部位进行检测和分析。结果]细叶榕、垂叶榕和夹竹桃对脱水响应的结果表明:①叶片各测定部位之间的光合反应中心最大光能转换效率(Fv/Fm)和非光化学猝灭(NPQ)的值较小,而电子传递速率(ETR)、PSII真实光化效率(ΦPSII)、光化学猝灭(qP)则有较大的差异;②水分胁迫下叶片不同部位的这种离散性更加明显;③抗逆性强的离散程度明显变小。结论]为叶绿素成像技术进一步应用于植物对干旱胁迫的响应研究奠定了基础。

Response of Ficus microcarpa L.Foliage to Water Stress Determined by Chlorophyll Fluorescence Imaging Technique

Objective] This study was to determine the response of Ficus microcarpa L.foliage to polyethylene glycol(PEG) simulated water stress using chlorophyll fluorescence imaging technique.Method] The responses of detached leaves from Ficus microcarpa,Ficus benjamina and Nerium oleander to PEG-6000 simulated water stress were detected,and the chlorophyll fluorescence imaging technique was used to detect and analyze different spots of a single leaf simultaneously.Result] The responses of Ficus microcarpa,Ficus benjamina and Nerium oleander to dehydration showed that: ① the maximal photochemical efficiency(Fv/Fm) and non-photo-chemical quenching(NPQ) values were small in the reaction center among different detected spots of leaves,and there were great differences between relative electron transport rate(ETR),photochemical quenching(qP) and quantum efficiency of PSII photochemistry(ΦPSII);②the differences of these parameters were more obvious among different spots of water-stressed leaves;③ there was obviously little differences among species with strong resistance.Conclusion] This study lays foundation for the further research on the response of plants to drought stress using chlorophyll fluorescence imaging technique.