青蛤不同地理群体遗传结构差异的AFLP分析

目的]探讨青蛤种群遗传多样性水平和遗传分化特征。方法]采用AFLP技术对我国山东潍坊(WF)、江苏南通(NT)、浙江宁波(NB)、浙江温州(WZ)沿海共4个青蛤地理群体的遗传结构差异进行了分析。结果]5对引物共得到261个位点,4个群体的多态位点比例平均为82.95%,其中WZ群体最高(86.97%),NT群体最低(78.93%);4个群体Nei’s基因多样性指数分别为0.258 1、0.252 6、0.271 3和0.277 6,香农多样性指数分别为0.394 7、0.383 0、0.412 0和0.421 4;群体遗传变异主要来自于群体内,占96.39%;WF和NT群体、NB和WZ群体遗传关系较近,聚类分析分别先聚在一起。结论]研究的青蛤4个群体遗传基础较好,且尚未有明显的遗传分化。

AFLP Analysis of Genetic Structure of Cyclina sineusis.from Different Geographical Populations

Objective] The aim was to discuss the genetic diversity level and classification of Cyclina sineusis.Method] The genetic structure of four geographical populations from costal China of Weifang of Shandong(WF),Nantong of Jiangsu(NT),Ningbo of Zhejiang(NB),and Wenzhou of Zhejiang(WZ) were analyzed by using AFLP Markers.Result] The total 261 clear amplification bands were detected through five pairs of AFLP primer,the average rate of polimorphic loci was 82.95%,of which the greatest was WZ population(86.97%) and the lowest was NT population(78.93%).The gene diversity indexes of 4 populations of WF,NT,NB and WZ,the Nei were 0.258 1,0.252 6,0.271 3 and 0.277 6,and Shannon’s information index were 0.394 7,0.383 0,0.412 0 and 0.421 4.Genetic variations mainly came from individuals within populations(96.39%).WF and NT population,NB and WZ population were clustered together because of close genetic relation.Conclusion] The genetic heredity of Cyclina sineusis.was good and it showed indistinct heredity classification.