| 巢湖鸭Ghrelin基因外显子3的SNP检测及其与体尺性状的关联分析 |
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| 引用本文： | 李俊营,詹凯,许月英,赵瑞宏,唐中节.巢湖鸭Ghrelin基因外显子3的SNP检测及其与体尺性状的关联分析[J].安徽农业科学,2009,37(10):4443-4445. |
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| 作者姓名： | 李俊营 詹凯 许月英 赵瑞宏 唐中节 |
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| 作者单位： | 安徽省农业科学院畜牧兽医研究所,安徽合肥,230031;安徽省庐江县巢湖鸭保种场,安徽庐江,231490 |
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| 基金项目： | 安徽省“十一五”科技攻关计划资助项目(07010302132) |
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| 摘 要： | 目的]分析巢湖鸭Ghrelin基因多态性及其与巢湖鸭体尺性状的关系。方法]以巢湖鸭为试验材料,采用PCR-SSCP分析检测巢湖鸭Ghrelin基因外显子3的单核苷酸多态性,并将其与巢湖鸭体重、体斜长、胸深、胸宽和龙骨长等体尺性状进行关联分析。结果]检测到3种基因型AA、BB和AB。巢湖鸭外显子3第54位发生了G→A的突变,该突变位点对巢湖鸭体重、胸深和龙骨长的影响达到极显著水平（P〈0.01）,对体斜长、胸宽和半潜水长的影响达到显著水平（P〈0.05）,对胫长和胫围的影响不显著（P〉0.05）。BB基因型的体重、体斜长、胸深、胸宽、龙骨长和半潜水长显著高于AA型和AB型。结论]Ghrelin基因多态性与鸭的部分体尺性状存在显著相关性,且对B等位基因进行选择将有利于提高巢湖鸭的体尺指标。
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| 关 键 词： | Ghrelin基因 巢湖鸭 体尺性状 PCR-SSCP |
SNP Detection on the Polymorphism of Ghrelin Gene of Chaohu Duck and Association Analysis on its Polymorphism and Body Measurement Traits |
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| LI Jun-ying et al.SNP Detection on the Polymorphism of Ghrelin Gene of Chaohu Duck and Association Analysis on its Polymorphism and Body Measurement Traits[J].Journal of Anhui Agricultural Sciences,2009,37(10):4443-4445. |
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| Authors: | LI Jun-ying |
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| Institution: | LI Jun-ying et al(Institute of Animal Husb,ry , Veterinary,Anhui Academy of Agricultural Sciences,Hefei,Anhui 230031) |
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| Abstract: | Objective] The aim was to analyze the polymorphism of Ghrelin gene of Chaohu duck and the relationship with its body measurement traits.Method] With Chaohu duck as experimental material,the single nucleotide polymorphisms(SNPs) of exon 3 of Ghrelin genes from Chaohu duck were detected by PCR-SSCP analysis and the association analysis with its body measurement traits of body weight,body slanting length,chest depth and width,and keel length were made.Result] There were 3 kinds of genotypes of AA,BB and AB.... |
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| Keywords: | Ghrelin gene Chaohu duck Body measurement traits PCR-SSCP |
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