| 蜗牛酶中纤维素酶的分级分离 |
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| 引用本文： | 邬晓勇,何钢,孙雁霞,苟小军.蜗牛酶中纤维素酶的分级分离[J].安徽农业科学,2009,37(4):1394-1396. |
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| 作者姓名： | 邬晓勇 何钢 孙雁霞 苟小军 |
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| 作者单位： | 成都大学中药化学实验室,四川,成都,610106;成都大学中药化学实验室,四川,成都,610106;中国医药集团四川抗菌素工业研究所,四川,成都,610051 |
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| 摘 要： | 目的]探索用分级沉淀方法去除蜗牛酶中非纤维素酶的效果。方法]向粗酶液中加入硫酸铵，使溶液中的硫酸铵从10％饱和度以10％为梯度逐渐递增到100％的饱和度，用考马斯亮蓝法测定蛋白含量，DNS法测定纤维素酶活力。结果]硫酸铵饱和度达到40％时原酶液中的大部分非纤维素酶杂蛋白开始沉淀，但此时所得沉淀的比活力最低；在50％饱和度下分离出的沉淀中纤维素酶的活力最高，在50％及之后的几个饱和度时，分离出了较多的纤维素酶蛋白，表明硫酸铵分级沉淀蜗牛酶可以显著提高其中纤维素酶的活力。结论]用50％的饱和度分级沉淀蜗牛酶中的纤维素酶最合理。
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| 关 键 词： | 蜗牛酶 纤维素酶 分级沉淀 |
Grade Separation of Cellulase from Snailase |
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| WU Xiao-yong et al.Grade Separation of Cellulase from Snailase[J].Journal of Anhui Agricultural Sciences,2009,37(4):1394-1396. |
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| Authors: | WU Xiao-yong |
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| Institution: | WU Xiao-yong et al(Laboratory for Chemistry of Traditional Chinese Medicine,Chengdu University,Chengdu,Sichuan 610106) |
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| Abstract: | Objective]The aim was to explore the effect of using fractional precipitation to remove the non-cellulose from snailase.Method] Adding ammonium sulphate to crude enzyme,the saturation of ammonium sulphate in the solution was increased gradually from 10% to 100% with the gradient of 10%.The protein content was detected by the coomassie brilliant blue method and the cellulase activity was detected by DNS method.Result]The impure protein in most non-cellulose from snailase in the original enzyme liquid bega... |
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| Keywords: | Snailase Cellulose Fractional precipitation |
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