首页 | 本学科首页   官方微博 | 高级检索  
     检索      

菲律宾蛤仔酶解多肽抗前列腺癌DU-145细胞的活性研究
引用本文:李连军,徐律,杨最素,丁国芳,孙瑜.菲律宾蛤仔酶解多肽抗前列腺癌DU-145细胞的活性研究[J].安徽农业科学,2013,41(15):6717-6718,6762.
作者姓名:李连军  徐律  杨最素  丁国芳  孙瑜
作者单位:浙江海洋学院食品与医药学院,浙江省海洋生物医用制品重点工程技术研究中心,浙江舟山316004;浙江海洋学院食品与医药学院,浙江省海洋生物医用制品重点工程技术研究中心,浙江舟山316004;浙江海洋学院食品与医药学院,浙江省海洋生物医用制品重点工程技术研究中心,浙江舟山316004;浙江海洋学院食品与医药学院,浙江省海洋生物医用制品重点工程技术研究中心,浙江舟山316004;浙江海洋学院食品与医药学院,浙江省海洋生物医用制品重点工程技术研究中心,浙江舟山316004
摘    要:目的]探讨菲律宾蛤仔酶解多肽抗人前列腺癌DU-145细胞增殖的活性。方法]对菲律宾蛤仔内脏进行酶解,经超滤截取3 kD以下的多肽,应用MTT法、倒置显微镜观察和AO/EB染色等方法检测其体外抗DU-145细胞的活性。结果]MTT法检测结果表明不同浓度的多肽对DU-145细胞有明显的生长抑制作用。通过倒置显微镜观察和AO/EB荧光双染观察到DU-145细胞出现细胞凋亡的形态学特征。结论]菲律宾蛤仔3 kD以下的多肽具有抑制DU-145细胞生长,呈浓度和时间依赖性,并诱导细胞凋亡作用。

关 键 词:菲律宾蛤仔  酶解多肽  前列腺癌细胞  凋亡

Study on Antiproliferative Activity of Ruditapes philippinarum Hydrolysate on Human Prostate Cancer DU-145 Cell
Institution:LI Lian-jun et al(School of Food Science and Pharmacy of Zhejiang Ocean University,Zhejiang Provincial Key Engineering Technology Research Center of Marine Biomedical Products,Zhoushan,Zhejiang 316004)
Abstract:Objective] To study Philippines clam enzymolysis peptide on prostate cancer DU-145 cells proliferation activity in vitro.Method] Digestive enzyme was used to enzymolysis the Philippine clam guts,3 kD molecular weight cut-off membranes were used to fractionate the Ruditapes philippinarum hydrolysate.The anti-proliferation activity and the early apoptosis of Ruditapes philippinarum peptides in vitro were detected by MTT,inverted microscope and AO/EB double staining respectively.Result] Determined by MTT method,different concentration of peptides to DU-145 cells had obvious growth inhibition.Cell apoptosis morphological feature was observed under AO/EB double staining and inverted microscope.Conclusion] Ruditapes philippinarum under 3 kD enzyme solution of molecular weight with inhibition of DU-145 cells growth,induce prostate cancer cells apoptosis,and a concentration and time dependence.
Keywords:Ruditapes philippinarum  Hydrolyzed peptide  Prostate cancer cell  Apoptosis
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号