| 苎麻纤维中β-葡聚糖酶活力测定条件的比较研究 |
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| 引用本文： | 申素芳,晏春耕,曹瑞芳,谢政.苎麻纤维中β-葡聚糖酶活力测定条件的比较研究[J].安徽农业科学,2013,41(16):7051-7052,7056. |
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| 作者姓名： | 申素芳 晏春耕 曹瑞芳 谢政 |
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| 作者单位： | 湖南农业大学生物科学技术学院,湖南长沙,410128;湖南农业大学生物科学技术学院,湖南长沙,410128;湖南农业大学生物科学技术学院,湖南长沙,410128;湖南农业大学生物科学技术学院,湖南长沙,410128 |
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| 摘 要： | 目的]为了获得苎麻纤维β-葡聚糖活力测定的最佳条件,比较苎麻纤维和饲料添加剂中β-葡聚糖酶测定方法的异同点。方法]采用DNS法,对苎麻纤维中β-葡聚糖酶活性的测定条件进行比较研究。结果]测定苎麻纤维中β-葡聚糖酶活力的最佳条件为:50℃条件下反应30 min后沸水浴10 min。结论]该方法可以为苎麻纤维中β-葡聚糖酶的酶活力测定提供借鉴。
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| 关 键 词： | 苎麻 韧皮纤维 DNS法 β-葡聚糖酶 酶活力 |
Comparative Study of Determination Condition of β-Glucan Enzyme Activity in Ramie Fibre |
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| Institution: | SHENG Su-fang et ai (College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha,Hunan 410128) |
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| Abstract: | Objective] In order to get the optimum condition of the determination of the activity of β-glucan enzyme in the ramie fibre,the comparative analysis was carried out to study the similarities and differences between ramie fibre and feed supplement.Method]The determination conditions of β-glucan enzyme activity in ramie fibre were analyzed using DNS method.Result]The optimum condition of the determination of β-glucan enzyme activity was reacting 30 min in water whose temperature was 50 degrees,boiling water bath for 10 min.Conclusion] This method could provide a reference for the determination of β-glucan enzyme activity in the ramie fibre. |
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| Keywords: | Ramie Phloem fibre DNS method β-gulcan Enzyme activity |
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