| 磷化氢熏蒸对米象抗性相关基因表达的影响 |
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| 引用本文： | 刘畅,胡飞,鲁玉杰,魏兆军.磷化氢熏蒸对米象抗性相关基因表达的影响[J].安徽农业科学,2013,41(15):6881-6883. |
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| 作者姓名： | 刘畅 胡飞 鲁玉杰 魏兆军 |
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| 作者单位： | 1. 合肥工业大学生物与食品工程学院,安徽合肥,230009
2. 河南工业大学粮油食品学院,河南郑州,450001 |
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| 基金项目： | 植物病虫害生物学国家重点实验室开放基金项目，安徽省第四批优秀青年科技基金项目 |
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| 摘 要： | 目的]考察磷化氢熏蒸处理后米象抗性相关基因的表达量情况,为基因功能研究提供参考。方法]对米象成虫进行磷化氢熏蒸后,使用荧光定量PCR对谷胱甘肽硫转移酶基因家族、乙酰胆碱酯酶基因等10个特异性靶标酶基因的表达变化进行分析。结果]磷化氢熏蒸处理后,米象的谷胱甘肽硫转移酶相关基因的表达量总体呈上升趋势;抗性相关靶标基因的表达量总体呈现下降的趋势。结论]谷胱甘肽硫转移酶等基因均与抗性相关,研究可为今后明确米象抗性分子机制提供参考。
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| 关 键 词： | 米象 磷化氢 表达变化 |
Effects of Phosphine on the Expression of the Genes Related to the Insecticide Resistance of Sitophilus oryzae |
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| Institution: | LIU Chang et al(School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei,Anhui 230009) |
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| Abstract: | Objective] After the stress of PH3,research the expression of genes related to the insecticide resistance in Sitophilus oryzae and provide reference for gene function research.Method] After the stress of PH3,the expression of Glutathione S-transferase family genes and ten genes encoding of insecticide targets were analyzed.Result] The stress of PH3 can induce the increase of expression level of Glutathione S-transferase family genes and the decrease of genes of insecticide target.The extent of the increase or decrease depended on the different genes.Conclusion] Glutathione S-transferase family genes related to the insecticide resistance,which provided the foundation for elucidating the molecular mechanisms of resistance and control by biotechnology. |
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| Keywords: | Sitophilus oryzae Phosphine Expression variation |
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