紫蛇尾多糖的抗氧化和抑菌活性研究

目的]提取紫蛇尾粗多糖并研究其抗氧化及抑菌活性。方法]以紫蛇尾为材料,通过脱脂、脱蛋白后得到紫蛇尾多糖,采用Fenton反应和邻苯三酚自氧化法来测定不同浓度的多糖溶液对自由基的清除能力,用滤纸片法测定紫蛇尾多糖对大肠杆菌、金黄色葡萄球菌、啤酒酵母菌和青霉的抑制作用。结果与结论]紫蛇尾多糖具有较好的抗氧化及抑菌活性。

Study on Bacteriostasis and Anti-oxidation of Polysaccharides from Ophiopholis mirabilis

Objective] To prepare polysaccharedes from Ophiopholis mirabilis and analyze its anti-oxidation,bacteriostasis activity.Method] With Ophiopholis mirabilis as raw materials,polysaccharides were obtained by degreasing and deproteinizing.Fenton reaction and pyrogallol autoxidation method were adopted to determine scavenging free radical ability of different concentration of polysaccharide solution.The filter method was used to study inhibition effect on Escherichia coli,Staphylococcus aureus,Saccharomyces cerevisiae Hansen,Penicillium.Result] The polysaccharide from Ophiopholis mirabilis has preferable antioxidant and antibacterial activity.