| 不同小麦品种遗传多态性研究——基于小麦反转录转座子Wis2-1A的IRAP分析 |
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| 引用本文： | 郭向萌,周晓君,郑玲,押辉远. 不同小麦品种遗传多态性研究——基于小麦反转录转座子Wis2-1A的IRAP分析[J]. 安徽农业科学, 2010, 38(35): 19944-19945. DOI: 10.3969/j.issn.0517-6611.2010.35.023 |
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| 作者姓名： | 郭向萌 周晓君 郑玲 押辉远 |
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| 作者单位： | 洛阳师范学院生命科学系,河南洛阳,471022;洛阳师范学院生命科学系,河南洛阳,471022;洛阳师范学院生命科学系,河南洛阳,471022;洛阳师范学院生命科学系,河南洛阳,471022 |
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| 基金项目： | 河南省教育厅自然科学基金 |
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| 摘 要： | [目的]对不同小麦品种的遗传差异进行客观评估。[方法]提取21种小麦幼苗总DNA,利用基于小麦反转录转座子Wis2-1A的分子标记技术作IRAP分析,对其遗传多态性进行评价。[结果]电泳结果进行聚类分析,有19个品种聚成不同遗传距离的类群,绝大多数小麦品种得到了充分的区分,对不同品种的小麦遗传多样性进行了客观评价。[结论]基于小麦反转录转座子Wis2-1A的IRAP分析,可作为小麦育种判断遗传距离的依据。
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| 关 键 词： | 小麦 反转录转座子 IRAP 遗传多态性 |
Study on Genetic Polymorphism of Some Varieties of Wheats |
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| Affiliation: | GUO Xiang-meng et al(Life Sciences Department of Luoyang Normal University,Luoyang,Henan 471022) |
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| Abstract: | [Objective]To analyze genetic polymorphism of different species of wheat.[Method]The DNA of young seedlings from 21 species of wheat was isolated,and their genetic polymorphism was analyzed by inter-retrotransposon amplified polymorphism(IRAP) using a molecule marker technology based on wheat retrotransposon Wis2-1A.[Result]As shown by clustering map of the electrophoresis results,19 species of wheat assembled as clusters with different genetic distance.Most of the wheat species was distinguished.The genetic polymorphism among different species of wheat could be evaluated by this method objectively.[Conclusion]The analysis of IRAP based on wheat retrotransposon Wis2-1A could give a basis for breeding of wheat. |
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| Keywords: | Wheat Retrotransposon IRAP Genetic polymorphism |
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