| 枸杞nrDNA ITS测序鉴定的初步研究 |
| |
| 引用本文： | 石志刚,安巍,樊云芳,焦恩宁,赵建华,王亚军.枸杞nrDNA ITS测序鉴定的初步研究[J].安徽农业科学,2008,36(16):6687-6688. |
| |
| 作者姓名： | 石志刚 安巍 樊云芳 焦恩宁 赵建华 王亚军 |
| |
| 作者单位： | 宁夏枸杞工程技术研究中心,宁夏银川,750002;宁夏枸杞工程技术研究中心,宁夏银川,750002;宁夏枸杞工程技术研究中心,宁夏银川,750002;宁夏枸杞工程技术研究中心,宁夏银川,750002;宁夏枸杞工程技术研究中心,宁夏银川,750002;宁夏枸杞工程技术研究中心,宁夏银川,750002 |
| |
| 基金项目： | 宁夏自然科学基金资助项目 |
| |
| 摘 要： | 目的]对枸杞nrDNA ITS(核糖体DNA基因内转录间隔区)序列进行分析,从而在分子水平对枸杞做出鉴定。方法]采用改良CTAB法提取枸杞叶片DNA,利用合成的特异引物对其DNA中nrDNA ITS区进行扩增、克隆,对目的片段测序分析。结果]克隆到枸杞nrDNA ITS片段并获得其碱基序列,成功找到3个供试枸杞种质材料的nrDNA ITS序列差异。结论]枸杞nrDNAITS区的扩增和测序可以在分子水平对枸杞不同种质进行鉴别。
|
| 关 键 词： | 枸杞属 ITS序列 DNA测序 鉴定 |
| 文章编号： | 0517-6611(2008)16-06687-02 |
| 修稿时间： | 2008年3月24日 |
Preliminary Studies on Identification of Lycium Linn.Germplasm Resources by nrDNA ITS Sequencing |
| |
| SHI Zhi-gang.Preliminary Studies on Identification of Lycium Linn.Germplasm Resources by nrDNA ITS Sequencing[J].Journal of Anhui Agricultural Sciences,2008,36(16):6687-6688. |
| |
| Authors: | SHI Zhi-gang |
| |
| Abstract: | Objective] The study aimed to identify wolfberry (Lycium Linn.) germplasm resources at molecular level by analyzing the nrDNA ITS sequence.Method] Genomic DNAs from wolfberry leaves extracted by modified CTAB method were regarded as templates for PCR amplification by specific primer,clone and sequencing.Result] The nrDNA ITS sequences were obtained and then differentiated among three tested materials.Conclusion] PCR amplification and sequencing on nrDNA ITS is a feasible approach to identify different wolfberry germplasm resources. |
| |
| Keywords: | Lycium L ITS sequence DNA sequencing Identification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
