| 5个鸭品种生长激素基因座位遗传多态性检测 |
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| 引用本文： | 伍革民,郗正林,许晓风.5个鸭品种生长激素基因座位遗传多态性检测[J].安徽农业科学,2007,35(5):1300-1301. |
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| 作者姓名： | 伍革民 郗正林 许晓风 |
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| 作者单位： | 1. 南京师范大学生命科学学院,江苏南京,210097;云南农业大学动物科技学院,云南昆明,650201;2. 江苏省南京市畜牧家禽研究所,江苏南京,210036;3. 南京师范大学生命科学学院,江苏南京,210097 |
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| 摘 要： | 应用PCR-SSCP技术首次分析5个品种鸭生长激素基因座位5'端调控区、外显子与部分内含子和3'端调控区中的多态位点,对具有多态性的位点进行测序,以探讨鸭生长激素基因遗传变异情况,比较不同品种该基因座位遗传多样性差异.结果在5个品种鸭中共检测到5个多态位点,多态位点频率在5个不同品种鸭中存在明显差异.序列比较分析结果表明:鸭生长激素基因座位5'端与3'端调控区和外显子序列具有极高的保守性,在5个鸭品种间不存在任何差异,所测出的序列与已知鸭GH序列相应部分完全一致.检测出的6处突变点(包括4个替换、1个插入和1个缺失)均位于内含子区域.推测鸭生长激素基因表达差异可能受内含子序列影响.
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| 关 键 词： | 鸭 生长激素基因 PCR-SSCP |
| 文章编号： | 0517-6611（2007）05-01300-02 |
| 修稿时间： | 2006-11-20 |
DNA Polymorphism of Growth Hormone Gene in Five Duck Breeds |
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| WU Ge-min et al.DNA Polymorphism of Growth Hormone Gene in Five Duck Breeds[J].Journal of Anhui Agricultural Sciences,2007,35(5):1300-1301. |
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| Authors: | WU Ge-min |
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| Institution: | College of Life Science, Nanjing Normal University, Nanjing, Jiangsu 210097 |
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| Abstract: | DNA polymorphism of growth hormone gene in five duck breeds including 5’flanking,3’flanking and exon sequence were studied using PCR-SSCP.Five GH alleles were identified in five duck breeds.And their frequencies were significantly different between breeds.There were high conservatism in duck GH gene 5’flanking,3’flanking and exon sequence and no differences among the five breeds.Meanwhile,there were six mutational sites(4substitutions,1insertion and 1absence) in intron region. |
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| Keywords: | Duck Growth hormone(GH)gene PCR-SSCP |
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