| 纤维素分解细菌的分离和鉴定 |
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| 引用本文： | 李安市,耿学磊,贾晶,张小葵,陈冠军.纤维素分解细菌的分离和鉴定[J].安徽农业科学,2009,37(9):3956-3959. |
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| 作者姓名： | 李安市 耿学磊 贾晶 张小葵 陈冠军 |
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| 作者单位： | 山东大学威海分校海洋学院,山东威海,264209;山东大学微生物技术国家重点实验室,山东济南,250100 |
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| 基金项目： | 鸣谢研究得到威海市能源站的大力支持! |
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| 摘 要： | 目的]研究低温环境下纤维素降解细菌的分离与鉴定。方法]采用低温培养的方法从秸秆堆肥中筛选出3株分解纤维素的细菌,通过PCR克隆这3株菌的16SrDNA并与相似菌株做比对,进一步构建分子进化树,来研究其分类情况。结果]综合其个体形态、菌落形态、生理生化特征、16SrDNA发育树构建结果等分类依据,鉴定3株菌均为假单胞菌属（Pseudomonas）。结论]WH3为Pseudomonasputida,WH1和WH12还需进一步鉴定。
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| 关 键 词： | 纤维素降解细菌 分离 鉴定 |
Isolation and Identification of Efficient Cellulose-degrading Bacteria |
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| LI An-shi et al.Isolation and Identification of Efficient Cellulose-degrading Bacteria[J].Journal of Anhui Agricultural Sciences,2009,37(9):3956-3959. |
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| Authors: | LI An-shi |
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| Institution: | LI An-shi et al (Marine College of Sh,ong University at Weihai,Weihai,Sh,ong 264209) |
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| Abstract: | Objective]The research aimed to study the isolation and identification of efficient cellulose-degrading bacteria.Method]Three cellulose-degrading bacteria,WH1,WH3 and WH12 were isolated from the composts through selective culture conditions.To investigate the phylogenetic position of these strains,the 16S rDNA sequences were cloned,sequenced and compared with those of related strains.The molecular phylogenetic dendrogram were constructed based on genetic distance analysis.Result]Three strains were all id... |
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| Keywords: | Cellulose-degrading bacteria Isolation Identification |
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