葡萄皮总黄酮的提取及其对羟基自由基清除作用的研究

目的]优化葡萄皮总黄酮的提取工艺,并考察葡萄皮提取物对羟基自由基的清除效果。方法]以市售葡萄为原料,采用正交试验法对影响葡萄皮总黄酮提取率的主要因素进行研究和分析,考察了乙醇浓度、回流温度、回流时间及料液比4个因素对葡萄皮总黄酮提取率的影响,筛选出最佳工艺条件研究其提取物对羟基自由基的清除效果。结果]葡萄皮黄酮乙醇浸提最佳条件为乙醇浓度40%,浸提时间1 h,固液比1∶15 g/ml,浸提温度85℃,在此工艺条件下葡萄皮总黄酮含量为65.17 mg/g。葡萄皮提取物中含有对羟基自由基具有清除能力的有效成分,并有较好的清除效果。结论]该研究得到葡萄皮中总黄酮提取的最佳工艺,并为葡萄皮的药用价值提供科学依据。

Study on Extraction of Total Flavonoids from Grape Skin and Its Antioxidation Effects

Objective] To optimize the extraction process of flavonoids from grape skin and its effect to scavenge the hydroxyl free radicals.Method] With the grapes sold on market as materials,the main factors influencing the extraction rate of total flavonoids were analyzed by orthogonal experiment,the effects of four factors,including ethanol concentration,reflux temperature,reflux time and solid-liquid ratio,on the extraction rate of flavonoids were studied,and the best extraction conditions were found out.Result] The best extraction conditions were 40% of ethanol concentration,1 h of extraction time,1∶15(g/ml) of solid-liquid ratio and 85 ℃ of extraction temperature,under which the total amount of extracted flavonoids was 65.17 mg/g.The grape skin contained some effective components with the scavenging capacity of hydroxyl free radicals.Conclusion] The best extraction conditions of total flavonoids were obtained in this study,providing scientific basis for the medical value of grape skin.