| 鸭源大肠杆菌氟苯尼考耐药基因floR的流行调查 |
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| 引用本文： | 吴华,冯建坤,贺志沛,刘保光,胡功政.鸭源大肠杆菌氟苯尼考耐药基因floR的流行调查[J].安徽农业科学,2012,40(28):13828-13829,13834. |
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| 作者姓名： | 吴华 冯建坤 贺志沛 刘保光 胡功政 |
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| 作者单位： | 河南农业大学牧医工程学院,河南郑州,450002;河南农业大学牧医工程学院,河南郑州450002;西藏职业技术学院畜牧兽医系,西藏拉萨850030 |
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| 基金项目： | 国家自然科学基金项目(30771624) |
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| 摘 要： | 目的]了解我国部分地区鸭源大肠杆菌氟苯尼考耐药基因floR在临床菌株中的流行情况。方法]采用美国临床实验室标准化委员会(CLSI)推荐的肉汤微量稀释法测定22株鸭大肠杆菌对氟苯尼考等9种药物的敏感性,并应用PCR方法检测氟苯尼考耐药基因floR。结果]22株鸭源大肠杆菌对氟苯尼考和四环素全部耐药,耐药率达100%,呈现多重耐药性特点;其氟苯尼考耐药基因floR检出率为90.9%,与耐药表型基本一致。结论]氟苯尼考在我国部分地区呈现高耐药性,对其耐药基因的监测为临床抗菌药物的合理使用提供了理论依据。
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| 关 键 词： | 氟苯尼考 floR 耐药 PCR |
Detection of the floR Genes in Florfenicol-resistant Escherichia coli Isolated from Diseased Ducks |
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| Institution: | WU Hua et al(College of Animal Science and Veterinary Medicine,Henan Agricultural University,Zhengzhou,Henan 450002) |
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| Abstract: | Objective] To know the prevalence of floR genes in the clinical isolates of Escherichia coli from ducks in some areas in China.Method] 22 strains of E.coli were isolated from sick ducks.Their MICs against 9 antibacterial agents were determined by the two-fold broth microdilution method according to Clinical Laboratory Standardization Institute(CLSI) and floR genes were amplified by PCR using every strain as template.Result] 22 strains of E.coli all exhibited resistance to florfenicol and tetracycline and owned multiple resistant phenotype.The detection rate of floR genes was 90.9%,which was in accordance with the resistance phenotype.Conclusion] floR resistant gene exhibited high drug resistance in some areas of China,through the monitoring of its drug resistance genes,some theoretical basis was provided for the reasonable application of clinical antibacterial drugs. |
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| Keywords: | Florfenicol floR Drug resistance PCR |
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