低能离子注入诱变选育盐霉素高产菌株

目的]研究N+离子注入对白色链霉菌的诱变效应,同时筛选高产盐霉素的变异菌株。方法]利用不同剂量的氮离子对白色链霉菌S-11-04菌株进行诱变处理,研究低能氮离子注入对其存活率、菌落形状及产盐霉素能力的影响。结果]低能氮离子注入对白色链霉菌的诱变效应显著,试验得到了13株盐霉素产量较高的突变菌株,其中N3-6菌株经连续传代4次,遗传稳定性较好,其摇瓶发酵水平较对照提高了41%,发酵生产后平均发酵水平提高了20.5%。结论]离子注入诱变是获得高产盐霉素突变菌株的有效方法。

Breeding of High-Yield Salinomycin-Producing Streptomyces albus Strain by Low Energy N~+ Ion Beam Irradiation

Objective] This study aimed to explore the mutagenesis effects of N+ ion beam irradiation on Streptomyces albus and obtain high-yield salinomycin-producing strain.Method] Streptomyces albus strain S-11-04 was mutated with different doses of N+ implantation.The effects of low energy N+ implantation on the survival rate,colony shape and salinomycin-producing ability were investigated.Result] The results showed that low energy N+ implantation can efficiently improve the positive mutation rate of Streptomyces albus;13 mutant strains with high yield of salinomycin were isolated;mutant strain N3-6 has good inheritance and fermentation stability with four continuous generations,and the titres of salinomycin were increased by 41% in the shake-flask culture and 20.5% in mass production compared with the control.Conclusion] N+ ion beam irradiation is an effective method to obtain high-yield salinomycin-producing Streptomyces albus strain.