GC-TOFMS外标法测定烟丝中烟碱含量 |
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引用本文: | 陈玉,陆舍铭,朱丽,孟昭宇,丁中涛.GC-TOFMS外标法测定烟丝中烟碱含量[J].安徽农业科学,2012(30):14986-14987,15074. |
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作者姓名: | 陈玉 陆舍铭 朱丽 孟昭宇 丁中涛 |
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作者单位: | 红塔烟草集团有限责任公司技术中心;云南大学化学科学与工程学院分析化学 |
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摘 要: | 目的]探讨GC-TOFMS外标法测定烟丝中烟碱含量的优选条件,建立一种快速测定烟碱的方法。方法]采用GC-TOFMS外标法测定烟丝中烟碱含量,分别对萃取溶剂、萃取方式、时间进行优化。结果]GC-TOFMS外标法测定烟丝中烟碱的最优溶剂是45 ml乙醇+5 ml 0.1%乙酸水溶液,振荡萃取,时间45 min。烟碱含量与峰面积比具有良好的线性关系,其相关系数r=0.996 6,烟碱的RSD为1.17%~1.54%(n=6),平均回收率为98.59%~101.03%。结论]该方法准确、可靠,运用该方法能快速测定烟丝中的烟碱。
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关 键 词: | 烟丝 烟碱 外标法 GC-TOFMS |
Determination of Nicotine in Cut Tobacco with External Standard Method by GC-TOFMS |
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Institution: | CHEN Yu et al(R&D Center of Hongta Tobacco Group Co.Ltd.,Yuxi,Yunnan 653100) |
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Abstract: | Objective] To explore optimization conditions for the determination of nicotine in cut tobacco by using GC-TOFMS and establish a rapid determination method.Method] The nicotine content on cut tobacco was determined by GC-TOFMS method.The extraction solvent,methods,time were optimized.Result] The optimum condition was extracted with mixture of 45 ml ethanol + 5 ml 0.1% acetic acid solution,oscillating for 45 min.The relationship between quantity of nicotine and peak area showed a good linear relation,the correlation coefficient was r=0.996 6.The RSD of nicotine was 1.17%-1.54%(n=6),the recovery rate was 98.59%-101.03%.It was proved that the method was accurate,reliable and suited to determine the content of nicotine in cut tobacco rapidly. |
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Keywords: | Cut tobacco Nicotine External standard method Gas chromatography time-of-flight mass spectrometry(GC-TOFMS) |
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