| 资木瓜多糖的提取和含量测定 |
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| 引用本文： | 李芳,李世刚,唐永莉,向正宗,陈燕.资木瓜多糖的提取和含量测定[J].安徽农业科学,2012,40(29):14197-14198,14278. |
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| 作者姓名： | 李芳 李世刚 唐永莉 向正宗 陈燕 |
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| 作者单位： | 三峡大学医学院,湖北宜昌,443002;三峡大学医学院,湖北宜昌,443002;三峡大学医学院,湖北宜昌,443002;三峡大学医学院,湖北宜昌,443002;三峡大学医学院,湖北宜昌,443002 |
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| 基金项目： | 湖北省教育厅项目(B20101201);宜昌市科技局项目(2010A01301-04);三峡大学科研基金项目(0620080702) |
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| 摘 要： | 目的]建立资木瓜多糖的提取和含量测定方法。方法]采用乙醇回流提取法提取资木瓜中的多糖,并利用苯酚-硫酸法测定多糖的含量。结果]线性回归方程为:Y=0.008 8X-0.010 9(R2=0.992 3)。试验结果表明,在1.0～5.0μg/ml范围内,葡萄糖浓度与吸光度的线性关系良好。提取2次后,脱脂木瓜中多糖的含量为13.21%;未脱脂木瓜中多糖的含量为9.26%;平均回收率为97.04%,RSD为3.52%。结论]该方法简便易行,稳定性、精密度和重现性良好,可用于资木瓜中多糖的提取和含量测定。
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| 关 键 词： | 资木瓜(FRUCTUS CHAENOMELIS) 多糖 提取 含量测定 |
Extraction and Determination of Polysaccharide from Chaenomeles speciosa |
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| Institution: | LI Fang et al(Medical College,China Three Gorges University,Yichang,Hubei 443002) |
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| Abstract: | Objective] To establish a method for the extraction and determination of polysaccharide from Chaenomeles speciosa.Method] Polysaccharides were extracted by the ethanol reflux method from Chaenomeles speciosa,and then determined by the method of C6H5OH phenol-H2SO4.Result] The linear regression equation was Y=0.008 8X-0.010 9(R2=0.992 3).As indicated by the results,the glucose concentration showed good linear relations with the absorbency within the range of 1.0-5.0 μg/ml.As twice of extraction,the content of polysaccharides was about 13.21% in skimmed Chaenomeles speciosa,and 9.26% in non-skimmed Chaenomeles speciosa.The average recovery was 97.04% with RSD of 3.52%.Conclusion] This method is simple with high stability,precision and reproducibility,which can be used for the extraction and determination of polysaccharides from Chaenomeles speciosa. |
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| Keywords: | Chaenomeles speciosa Polysaccharide Extraction Determination |
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