五倍子鞣质对生物被膜型白假丝酵母的干预作用

目的]研究五倍子鞣质对生物被膜型白假丝酵母的抑制作用。方法]在生物被膜形成的早期、中期和成熟期以不同浓度药物干预48 h,采用MTT法检测药物对膜型白假丝酵母的抑制率;以不同浓度药物干预成熟生物被膜24、48、72 h,再用MTT法检测抑制率;光镜直接观察生物被膜内白假丝酵母的形态结构变化;用吖啶橙/溴化乙锭染色,通过荧光显微镜观察生物被膜内白假丝酵母的死亡方式。结果]五倍子鞣质对生物被膜的形成具有抑制作用,对成熟期生物被膜中白假丝酵母的抑制作用具时间和剂量依赖性;鞣质导致被膜内白假丝酵母细胞变形,形态结构改变,但未见确切细胞凋亡,同时抑制细胞芽管和假菌丝形成。结论]五倍子鞣质对白假丝酵母生物被膜的形成及成熟期生物被膜中白假丝酵母均具较强的抑制作用;可能通过非凋亡途径导致被膜内白假丝酵母的死亡。

Intervention Effects of Tannins from Galla chinensis against Candida albicans in Biofilm

Objective] To study the inhibition action of tannins from Galla chinensis against Candida albicans in biofilm in vitro.Method] At early stage,middle stage and mature stage of biofilm formation,Candida albicans biofilm was incubated with different concentrations of tannins extracts for 48 h,and mature Candida albicans biofilm was incubated with different concentration of tannins extracts for 24,48 and 72 h,the cells inhibition rate was tested by MTT method.The morphology of Candida albicans in biofilm was observed by light microscope and the death mode was observed in the staining of AO/EB by fluorescence microscope.Result] Tannins from Galla chinensis could inhibit biofilm formation and mature biofilm,and the inhibition was time-and-dose-dependent during the mature stage.Tannins extracts led to the biofilm deformation,morphology and structure change of Candida albicans,but without obvious apoptosis.The tannins also inhibited the budding and formation of pseudohypha.Conclusion] Tannins from Galla chinensis could efficiently inhibit biofilm information and mature biofilm,and possibly lead to Candida albicans death by non-apoptosis pathway.