盐胁迫对大花金鸡菊生长及生理的影响

目的]研究盐胁迫对大花金鸡菊(Coreopsis grandiflora)生长和生理的影响,为大量栽培应用大花金鸡菊,加快其在园林绿化中的应用步伐提供理论依据,并为盐碱地绿化提供适宜的材料。方法]以大花金鸡菊幼苗作为试验材料,用浓度为0.2%、0.6%、1.0%NaCl、Na2SO4在其幼苗生长过程中进行胁迫,调查盐胁迫对幼苗生长的影响,并测定盐胁迫过程中叶片中蛋白质含量、可溶性糖含量、光合色素含量、丙二醛(MDA)含量、过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性、脯氨酸含量变化。结果]盐胁迫抑制了大花金鸡菊的生长;大花金鸡菊叶片中脯氨酸含量随处理浓度的增加而增加;高浓度盐处理使POD活性升高;NaCl处理下,SOD活性升高,但Na2SO4处理下低于对照;盐胁迫下,蛋白质、可溶性糖、丙二醛含量变化不显著,光合色素变化规律不明显。结论]大花金鸡菊具有一定抗盐性。

Effects of Salt Stress on Growth and Physiology of Coreopsis grandiflora

Objective] The aim was to study the effects of salt stress on growth and physiology of Coreopsis grandiflora,and provide a theoretical basis for application of Coreopsis grandiflora in a large scale and accelerating its application pace in landscape afforestation,and provide suitable planning materials for kaline soil.Method] The seedling of Coreopsis grandiflora were treated by NaCl and Na2SO4 respectively at the concentration of 0.2%,0.6% and 1.0%,the effects of salt stress on the seedlings growth,the changes of protein,soluble sugar,photosynthetic pigments,malondialdehyde and proline contents,and activity of POD and SOD in leaves were determined.Result] The results showed that the salty stress inhibited the seedlings growth.Proline content increased with the increasing salt concentration.POD activity were increased at high salt concentrations.SOD activity was increased under NaCl treatment,but lower than the control when treated by Na2SO4.Under NaCl and Na2SO4 stress,the changes of protein,soluble sugar and malondialdehyde contents were not significant,and the change of photosynthetic pigments had no obvious regularity.Conclusion]Coreopsis grandiflora has a certain degree of salt resistance through adjusting osmotic stress mainly by increasing proline content.