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太子参药材总DNA提取研究
引用本文:王晓多,王晓玲,王晓丽.太子参药材总DNA提取研究[J].安徽农业科学,2012,40(29):14167+14178.
作者姓名:王晓多  王晓玲  王晓丽
作者单位:贵阳市花溪区农业局,贵州贵阳,550025;贵州大学,贵州贵阳,550025;贵阳医学院药用植物与生药学教研室,贵州贵阳,550004
摘    要:目的]从太子参干药材中快速提取高质量基因组DNA。方法]采用CTAB法和SDS法2种方法提取药材太子参干燥块根总DNA,并用紫外分光光度计和琼脂糖凝胶电泳检测。结果]2种不同的提取方法均可从太子参中提取到一定纯度的DNA,其中CTAB法提取DNA的A260/A280>1.9,纯度和产率较高,蛋白质、多糖、RNA等杂志去除较为彻底。结论]利用CTAB法可从太子参干药材中能快速提取到高质量DNA。

关 键 词:太子参  DNA  吸光度值

Extraction of Genome DNA from Medicinal Materials of Pseudostellaria heterophylla(Miq.)Pax ex Pax et Hoffm.
Institution:WANG Xiao-duo et al(Huaxi Agricultural Bureau,Guiyang,Guizhou 550025)
Abstract:Objective] To extract high quality DNA from medicinal materials of Pseudostellaria heterophylla(Miq.)Pax ex Pax et Hoffm..Method] CTAB and SDS were used to extract DNA,the obtained DNA was examined with ultraviolet spectrophotometer and agarose gel electrophoresis.Result] The purity of the extracted DNA was very high with A260/A280>1.9 by the CTAB method,and the protein,polysaccharose and RNA were eliminated completely.Conclusion] The CTAB method was suitable for extracting DNA from medicinal materials of Pseudostellaria heterophylla(Miq.)Pax ex Pax et Hoffm..
Keywords:Pseudostellaria heterophylla(Miq  )Pax ex Pax et Hoffm    DNA  OD
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