| 东北刺人参愈伤组织诱导和继代的研究 |
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| 引用本文： | 葛江丽,李成浩,刘芳,施汉玉,栾泰龙,刘瑰琦,郑焕春,崔巍.东北刺人参愈伤组织诱导和继代的研究[J].安徽农业科学,2012(30):14660+14667. |
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| 作者姓名： | 葛江丽 李成浩 刘芳 施汉玉 栾泰龙 刘瑰琦 郑焕春 崔巍 |
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| 作者单位： | 黑龙江省牡丹江林业科学研究所;东北林业大学 |
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| 基金项目： | 黑龙江省森林工业总局青年基金项目(sgzjQ2010001) |
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| 摘 要： | 目的]研究东北刺人参愈伤组织的诱导和继代方法。方法]以取自长白山的刺人参植株为材料,研究不同外植体、培养基、激素对刺人参愈伤组织诱导的影响。结果]叶片为刺人参组织培养的最适宜外植体;5月为外植体的最佳取材时期;以MS为基本培养基,并添加1.0 mg/L 2,4-D和1.0 mg/L 6-BA的培养基中愈伤组织诱导率最高;最佳继代培养基为MS+6-BA 2.0 mg/L+NAA 0.2mg/L。结论]东北刺人参的最适愈伤诱导培养基和继代培养基分别是:MS+2,4-D 1.0 mg/L+6-BA 1.0 mg/L和MS+6-BA 2.0 mg/L+NAA 0.2 mg/L。
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| 关 键 词： | 东北刺人参 组织培养 愈伤组织 诱导条件 继代 |
Studies on the Callus Inducement and Subculture of Oplopanax elaus Nakai |
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| Institution: | GE Jiang-li et al(Mudanjiang Forestry Research Institute,Mudanjiang,Heilongjiang 157009) |
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| Abstract: | Objective] To study the callus induction and subculture method of Oplopanax elaus.Method] With O.elaus as material,the effect of different explants,mediums and hormones on callus induction was studied.Result] The results showed that leaf was the best suitable organs for tissue culture.May was the best period to draw materials.MS added to 2.0 mg/L 2,4-D and 1.0 mg/L 6-BA had high induction rate on callus;and the optimal subculture medium was MS+6-BA 2.0 mg/L+ NAA 0.2 mg/L.Conclusion] The best callus induction medium and ubculture medium of O.elaus: MS+2,4-D 1.0 mg/L+6-BA 1.0 mg/L and MS+6-BA 2.0 mg/L+NAA 0.2 mg/L. |
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| Keywords: | Oplopanax elaus Nakai Tissue culture Callus Inducement conditions Subculture |
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