河南省猪等孢球虫的分子变异规律研究

目的]掌握河南省猪等孢球虫的分子变异规律。方法]从河南省豫东、豫南、豫西、豫北及豫中地区的部分规模化猪场采集粪便样品,进行猪球虫卵囊的分离与纯化;从分离到的8株猪球虫分离株中提取核酸并进行测序,并与猪等孢球虫扩增株基因序列及其他相关原虫基因序列进行比对。结果]河南省不同地区的8个猪球虫株均为猪等孢球虫,并且没有明显遗传差异。I.suis与其他7种原虫差异较大,不属于同一个生物型,同一种动物所感染的不同种类原虫之间的同源性较低。结论]该研究结果可为有效预防和控制仔猪球虫病提供科学依据。

Study on the Molecular Variation Laws of Isospora suis in Henan Province

Objective] To master the molecular variation laws of Isospora suis in Henan Province.Method] The pig manure samples were collected from some large-scale pig farms in eastern,western,southern,northern and central regions of Henan Province to isolate and purify porcine coccidian oocysts.8 isolated strains of swine coccidiosis were used to extract nucleic acid and sequence.The gene sequence of I.suis were compared with that of other related protozoon.Result] 8 swine coccidian strains from different regions of Henan Province were identified as I.suis and there was no obvious genetic differences.I.suis had greater differences with other 7 kinds of protozoon.I.suis and other 7 kinds of protozoon belonged to different biotypes.The homology of different kinds of protozoon infected in the same kinds of animals was lower.Conclusion] The research results can provide scientific basis for effective prevention and control of piglet coccidiosis.