基于ISSR标记的20种荷花品种资源遗传多样性分析

目的]研究20种荷花材料遗传多样性和亲缘关系。方法]利用ISSR-PCR体系筛选出16条多态性引物,并利用16条ISSR引物对20种荷花材料进行PCR扩增。结果]16条引物扩增出225个位点,多态性位点占75.56%。通过Popgene32软件计算出20个荷花品种间的相似性系数为0.577 8~0.951 1,平均值为0.779 6。通过聚类分析将20个荷花品种分为两大类,白洋淀红莲和冬瓜莲可归为一类,其他18个品种划分为第二类。结论]ISSR标记技术可有效应用于不同荷花品种的遗传多样性分析和亲缘关系的鉴定。

Genetic Diversity of 20 Nelumbo nucifera Gaertn.Varieties Revealed by ISSR Analysis

Objective]To study the genetic diversity of 20 species of plant samples in Nelumbo nucifera Gaertn..Method]16 primers were screened by using ISSR amplification system,and the DNA was used to amplify of 20 species of plant samples with 16 primers.Result]16 primers produced 225 loci, the percentage of polymorphic loci was up to 75.56%.The genetic similarity coefficient of 20 species in squash ranged from 0.577 8 to 0.951 1,which was calculated by Popgene32.20 varieties by UPMGA analysis could be clustered into two groups,the first group included Baiyangdianhonglian and Donggualian;the rest of varieties was included in second group.Conclusion]ISSR molecular markers could be effectively used in genetic diversity and fingerprint analysis for 20 species of plant samples in Nelumbo nucifera Gaertn..