| 柴胡多糖的分子量测定及单糖组成分析 |
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| 引用本文： | 颜军,刘嵬,邬晓勇,郭晓强,何钢,张良蕾,孙晓春,苟小军.柴胡多糖的分子量测定及单糖组成分析[J].安徽农业科学,2010,38(9):4550-4552. |
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| 作者姓名： | 颜军 刘嵬 邬晓勇 郭晓强 何钢 张良蕾 孙晓春 苟小军 |
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| 作者单位： | 成都大学中药化学实验室,四川成都,610106;成都大学药用微生物重点实验室,四川成都,610106 |
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| 基金项目： | 四川省中医药管理局科技专项资助项目(2008-01);;四川省教育厅科研项目资助(08ZA173) |
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| 摘 要： | 目的]从柴胡中提取分离柴胡精多糖（BPP2）,并对其分子量和单糖组成进行研究。方法]采用水提法提取柴胡精多糖（BP）,用DEAE纤维素柱色谱进行分离纯化,采用GPC法测定BPP2的分子量,TLC、HPLC法对其单糖组成进行研究。结果]BPP2的分子量约为67836,由鼠李糖（Rha）、木糖（Xyl）、阿拉伯糖（Ara）、半乳糖（Gal）和葡萄糖（Glc）5种单糖组成,其摩尔比为2.19∶1.00∶3.21∶2.27∶2.44。结论]柴胡多糖BPP2是由5种单糖组成的纯一杂多糖,分子量为67836。
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| 关 键 词： | 柴胡多糖 分子量 单糖 组成 |
Molecular Weight Determination and Monosaccharide Composition Analysis of Bupleurum chinense DC Polysaccharide |
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| YAN Jun et al.Molecular Weight Determination and Monosaccharide Composition Analysis of Bupleurum chinense DC Polysaccharide[J].Journal of Anhui Agricultural Sciences,2010,38(9):4550-4552. |
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| Authors: | YAN Jun |
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| Institution: | Laboratory for Chemistry of Traditional Chinese Medicine;Chengdu University;Chengdu;Sichuan 610106 |
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| Abstract: | Objective] The aim was to extract and isolate Bupleurum chinense DC polysaccharide (BPP2),and study its molecular weight and monosaccharide composition. Method] BPP2 was extracted from B. chinense with water,isolated and purified by DEAE ion-exchange cellulose. The molecular weight of BPP2 was measured using GPC method,and TLC,HPLC were adopted to study its monosaccharide composition. Result] The molecular weight of BPP2 was 67 836,which was composed of Rha,Xyl,Ara,Gal and Glc with molar ratio of 2.19∶1.00∶3.21∶2.27∶2.44. Conclusion] BPP2 is pure heterosaccharide which is composed of five monosaccharides,the molecular weight is 67 836. |
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| Keywords: | Bupleurum chinense DC polysaccharide Molecular weight Monosaccharide Composition |
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