羊栖菜硫酸多糖的抗凝血活性研究

目的]探讨羊栖菜硫酸多糖的抗凝血活性。方法]从水提液中提取羊栖菜硫酸多糖,通过体内和体外的抗凝血试验系统地研究其抗凝血活性。结果]利用葡聚糖凝胶G-75分离纯化所提取的羊栖菜硫酸多糖F1,得到5个不同组分。在2.0 g/L的受试条件下,组分F12抗凝血活性的最强,其延长小白鼠的出血时间和凝血时间、大白兔的凝血活酶时间、凝血酶时间、凝血酶原时间分别为粗多糖F0的174.2%、153.7%、311.9%1、57.8%和218.2%。羊栖菜硫酸多糖F1与抗凝血效果有显著的量效关系。在受试条件下(浓度为0.5~2.0g/L),多糖的浓度与小白鼠的抗凝血指标、与大白兔体外抗凝血指标之间均呈现良好的直线回归关系。结论]该研究为羊栖菜类保健品及临床药物的开发提供理论基础。

Study on Anticoagulant Activity of Sulfated Polysaccharides from Hizikia fusiformis

Objective] The aim was to discuss on the anticoagulant activity of sulfated polysaccharides from Hizikia fusiformis.Method] The sulfated polysaccharides from H.fusiformis were extracted in water extract and their anticoagulant activities were studied systemically through the in vivo and in vitro test of anticoagulant activity.Result] The sephadex G-75 was used to separate and purify the extracted sulfated polysaccharides F1 from H.fusiformis to obtain 5 different fractions.The fraction F12 showed the highest anticoagulant activities under the concn.of 2.0 g/L.its bleeding time(BT) and clotting time(CT) of small white mice,activated partial thromboplastin time(APTT),thrombin time(TT) and prothrombin time(PT) of big white rabbit were prolonged by 174.2%,153.7%,311.9%,157.8% and 218.2% compared with the raw sulfated polysaccharides,resp.The dose-effect relationship was found between the quantity of sulfated polysaccharides F1 from H.fusiformis and the anticoagulant activities.Under the concn.of 0.5～2.0 g/L,the anticoagulant concn.had good linear regression relation with the anticoagulant indexes(BT and CT) of small white mice and the in vitro anticoagulant indexes(APTT,TT and PT) of big white rabbit.Conclusion] This study proviced theoretical base for developing the health products and clinical drugs from H.fusiformis.