合肥野生动物园袋鼠口鼻腔带菌情况调查

目的]对合肥野生动物园袋鼠口腔和鼻腔的带菌情况进行调查。方法]从袋鼠口腔和鼻腔中分离致病菌,并采用细菌形态学和分子生物学方法对其进行鉴定。选用14种常用抗菌药物,采用纸片扩散法进行药敏试验。结果]14份袋鼠口腔样本中均分离到革兰氏阳性球菌,其中12只袋鼠口腔分离到葡萄球菌,占85.7%;从4只袋鼠口腔分离到链球菌,占28.5%;从2只袋鼠口腔同时分离到葡萄球菌和链球菌,占14.3%。同时,从14只袋鼠鼻腔中均分离到葡萄球菌。药敏试验结果表明,ZS-1菌株对磺胺类、大环内酯类、喹诺酮类、头孢菌素类、磷霉素类、林可霉素类和四环素类药物敏感。鼻腔中细菌对磷霉素类、喹诺酮类和四环素类药物敏感。口腔中的细菌对14种常用抗菌药物均无耐药性,鼻腔中分离的细菌对卡那霉素、复方新诺明、强力霉素和阿莫西林耐药。结论]研究结果可为袋鼠口腔和鼻腔细菌感染类疾病的有效防治提供科学依据。

Investigation on the Infection Situations of Bacteria in Oral Cavity and Nasal Cavity of Kangaroo

Objective] To investigate the infection situations of bacteria in oral cavity and nasal cavity of kangaroo.Method] The pathogen were isolated from the oral cavity and nasal cavity of kangaroo and the isolated strains were identified by using the methods of bacterial morphology identification and molecular biology.14 kinds of antibacterial drugs were used to make the drug sensitivity test by disk diffusion method.Result] Gram-positive cocci were isolated from 14 oral cavity samples of kangaroo.Among them,staphylococcus were isolated from the oral cavity samples of 12 kangaroos,which took for 85.7%;streptococcus were isolated from the oral cavity samples of 4 kangaroos,which took for 28.5%.Staphylococcus and streptococcus were simultaneously isolated from the oral cavity samples of 2 kangaroos,which took for 14.3%.And staphylococcus were isolated from the nasal cavity of 14 kangaroos.The drug sensitivity results showed that ZS-1 strain was sensitive to sulfonamides,macrolides,quinolones,cephalosporins,fosfomycin,lincomycin and tetracycline.The isolated pathogen from the nasal cavity were sensitive to fosfomycin,quinolones and tetracycline.The isolated pathogen from the oral cavity had no tolerance to 14 kinds of common antibacterial drugs.The isolated pathogen from the nasal cavity were tolerant to kanamycin,sulfamethoxazole,doxycycline and amoxicillin.Conclusion] The research results can provide scientific basis for effective prevention and control of bacteriosis in oral cavity and nasal cavity of kangaroo.