| QuEChERS-液质联用法测定水果蔬菜中氯吡脲残留 |
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| 引用本文： | 胡江涛,俞凌云,帅培强,于刚,何开蓉.QuEChERS-液质联用法测定水果蔬菜中氯吡脲残留[J].安徽农业科学,2017,45(17). |
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| 作者姓名： | 胡江涛 俞凌云 帅培强 于刚 何开蓉 |
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| 作者单位： | 四川出入境检验检疫局检验检疫技术中心,四川成都,610041;四川出入境检验检疫局检验检疫技术中心,四川成都,610041;四川出入境检验检疫局检验检疫技术中心,四川成都,610041;四川出入境检验检疫局检验检疫技术中心,四川成都,610041;四川出入境检验检疫局检验检疫技术中心,四川成都,610041 |
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| 基金项目： | 四川省科技支撑计划项目，四川省卫生厅项目 |
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| 摘 要： | 目的]采用快速提取-高效液相色谱-串联质谱法(Qu ECh ERS-HPLC-MS/MS)建立水果蔬菜中氯吡脲残留的检测方法。方法]样品经含0.1%乙酸的乙腈溶液提取,经Qu ECh ERS法净化后采用HPLC-MS/MS法测定其氯吡脲残留量。结果]该方法下氯吡脲在水果蔬菜中的检出限(LOD)为1.0μg/kg,定量限(LOQ)为5.0μg/kg。在2.0~100.0μg/L范围内线性关系良好(R~20.999)。在10.0~500.0μg/kg的添加水平上,方法的平均回收率介于72.0%~115.0%,方法的相对标准偏差为1.5%~9.8%。结论]该方法适用于水果蔬菜中氯吡脲的测定。
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| 关 键 词： | 检测 氯吡脲 水果蔬菜 高效液相色谱-串联质谱法 |
Determination of Forechlorfenuron Residue in Fruits and Vegetables by QuEChERS Extraction and HPLC-MS/MS |
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| Abstract: | Objective] A quick extraction method QuEChERS-HPLC-MS/MS was established to determine forechlorfenuron in fruits and vegetables.Method] Fruits and vegetables were extracted with 0.1％ acetic acid of acetonitrile solution and purified by QuEChERS,then forechlorfenuron residues were determined by HPLC-MS/MS.Result] The limits of detection (LODs) and low determination limit (LOQ) for the forechlorfenuron was 1.0 μg/kg and 5.0 μg/kg in fruits and vegetables,respectively.Regression equations of these hormones had a good linear relationship (R2 ＞ 0.999) within 2.0-100.0 μg/L.The average recoveries of forechlorfenuron was in the range of 72.0％-115.0％with the coefficients of variation between 1.5％ and 9.8％ at the spiked levels of 10.0-500.0 μg/kg.Conclusion] The method can be applied for the determination of the forechlorfenuron in fruits and vegetables. |
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| Keywords: | Determination Forechlorfenuron Fruits and vegetables HPLC-MS/MS |
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