费菜总黄酮分离纯化方法研究 |
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引用本文: | 薛志忠,杨雅华,张国新,张薇.费菜总黄酮分离纯化方法研究[J].安徽农业科学,2017,45(18). |
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作者姓名: | 薛志忠 杨雅华 张国新 张薇 |
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作者单位: | 河北省农林科学院滨海农业研究所,河北唐山 063299;河北省盐碱地绿化工程技术研究中心,河北唐山 063299;河北省农林科学院滨海农业研究所,河北唐山 063299;河北省盐碱地绿化工程技术研究中心,河北唐山 063299;河北省农林科学院滨海农业研究所,河北唐山 063299;河北省盐碱地绿化工程技术研究中心,河北唐山 063299;河北省农林科学院滨海农业研究所,河北唐山 063299;河北省盐碱地绿化工程技术研究中心,河北唐山 063299 |
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基金项目: | 河北省农林科学院基本科研业务费 |
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摘 要: | 目的]开发高纯度费菜总黄酮的分离纯化工艺。方法]以大孔树脂为载体,对上样量及层析条件进行优化,再进一步采用结晶的方法进行纯化。结果]选用聚合物纳米微球PS RPC-300作为最佳层析填料,洗脱速度为5 m L/min,洗脱剂为70%乙醇,上样量为5.0 g/kg填料,结晶溶剂为丙酮;在此优化条件下所得产品的纯度为95.1%。结论]此分离纯化方法简便可靠,分离效果好。
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关 键 词: | 费菜 总黄酮 聚合物纳米微球 分离纯化 |
Study on the Separation and Purification of Total Flavonoids from Sedum aizoon L. |
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Abstract: | Objective] The research aimed to develop the separation and purification processes of high-purity total flavonoids from Sedum aizoon L.Method]With the macroporous resin as the carrier,the loading volume and the chromatographic conditions were optimized,and then further purified by crystallization method.Result]Type of PS RPC-300 nanoscale polymer particles was selected as the best chromatography filler.The optimal conditions were as follows:the elution speed was 5.0 mL/min,70% ethanol was used as the eluant,the amount of sample was 5 g/kg filter,and acetone was used as the crystallization solvent.The purity of the final product was 95.1% under the optimal conditions.Conclusion]The separation and purification method is simple and reliable,and the separation effect is good. |
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Keywords: | Sedum aizoon L Total flavonoids Polymer nanospheres Separation and purification |
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