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正交法优选亮菌多糖脱色工艺研究
引用本文:黄纯,王小平,程海涛,姜晟.正交法优选亮菌多糖脱色工艺研究[J].安徽农业科学,2017,45(21).
作者姓名:黄纯  王小平  程海涛  姜晟
作者单位:中国药科大学,江苏南京,211196;中国药科大学,江苏南京,211196;中国药科大学,江苏南京,211196;中国药科大学,江苏南京,211196
摘    要:目的]建立亮菌多糖脱色的最佳方法。方法]以色素脱除率作为主要考察指标,通过单因素考察和正交法优化亮菌多糖脱色工艺。结果]D152树脂、45℃、p H 5.0为最佳脱色条件。结论]D152作用后色素脱除率高,糖保留率高,有益于下一步分离纯化。

关 键 词:脱色素  亮菌多糖  工艺优化  正交法

Study on Decoloration Technique of Armillariella Polysaccharide by Orthogonal Experiments
Abstract:Objective] The research aimed to establish the optimum method of decoloration about Armillariella polysaccharide.Method]Taking the decoloring ratio as main index,the optimum decoloring technique were established through single factorial test and orthogonal experiments.Result]The best method of decoloration were obtained as follows: D152 resins working in pH 5.0 and 45 ℃.Conclusion]The decoloring ratio of D152 and the reteion rate of polysaccharide are high,which is beneficial to the next step.
Keywords:Decoloration  Armillariella polysaccharide  Technology optimization  Orthogonal method
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