正交法优选亮菌多糖脱色工艺研究 |
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引用本文: | 黄纯,王小平,程海涛,姜晟.正交法优选亮菌多糖脱色工艺研究[J].安徽农业科学,2017,45(21). |
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作者姓名: | 黄纯 王小平 程海涛 姜晟 |
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作者单位: | 中国药科大学,江苏南京,211196;中国药科大学,江苏南京,211196;中国药科大学,江苏南京,211196;中国药科大学,江苏南京,211196 |
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摘 要: | 目的]建立亮菌多糖脱色的最佳方法。方法]以色素脱除率作为主要考察指标,通过单因素考察和正交法优化亮菌多糖脱色工艺。结果]D152树脂、45℃、p H 5.0为最佳脱色条件。结论]D152作用后色素脱除率高,糖保留率高,有益于下一步分离纯化。
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关 键 词: | 脱色素 亮菌多糖 工艺优化 正交法 |
Study on Decoloration Technique of Armillariella Polysaccharide by Orthogonal Experiments |
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Abstract: | Objective] The research aimed to establish the optimum method of decoloration about Armillariella polysaccharide.Method]Taking the decoloring ratio as main index,the optimum decoloring technique were established through single factorial test and orthogonal experiments.Result]The best method of decoloration were obtained as follows: D152 resins working in pH 5.0 and 45 ℃.Conclusion]The decoloring ratio of D152 and the reteion rate of polysaccharide are high,which is beneficial to the next step. |
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Keywords: | Decoloration Armillariella polysaccharide Technology optimization Orthogonal method |
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