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铜胁迫对绿豆幼苗生理指标的影响
引用本文:朱惠敏,梁靖,吴宇东,周丽萍.铜胁迫对绿豆幼苗生理指标的影响[J].安徽农业科学,2017,45(27).
作者姓名:朱惠敏  梁靖  吴宇东  周丽萍
作者单位:肇庆学院生命科学学院,广东肇庆,526000;肇庆学院生命科学学院,广东肇庆,526000;肇庆学院生命科学学院,广东肇庆,526000;肇庆学院生命科学学院,广东肇庆,526000
基金项目:广东大学生科技创新培育专项
摘    要:目的]研究铜胁迫对绿豆幼苗生理指标的影响。方法]以不同浓度的铜溶液为培养液,研究Cu2+对绿豆幼苗活性氧清除系统及叶绿素含量的影响。结果]当Cu2+浓度在0~70 mg/L梯度内,绿豆幼苗的SOD活性、POD活性和叶绿素均呈先上升后下降的趋势,当Cu2+浓度为50 mg/L时,SOD活性和POD活性为最大值,随着浓度增大,活性下降;低浓度Cu2+促进叶绿素的合成,当Cu2+浓度为20 mg/L时,绿豆幼苗叶绿素含量最高,随着浓度增大,叶绿素含量明显下降。结论]铜胁迫明显影响活性氧清除酶系统,导致绿豆幼苗活性氧代谢失调。

关 键 词:Cu2+  绿豆  幼苗  生理指标

Effects of Cu Stress on Physiological Indicators of Vigna radiata Seedlings
Abstract:Objective]Effects of Cu stress on physiological indicators of Vigna radiata seedlings.Method]For nutrient solution with different concentrations of Cu solution,this paper studied the effect of Cu2 +on active oxygen scavenging system and chlorophyll content of V.radiata seed-ling.Result]When the concentration of copper ions was in the range of 0~70 mg/L,the SOD activity,POD activity and chlorophyll content of the V.radiata seedlings appeared to be increased firstly and then decreased .When the concentration of copper ions was 50 mg/L,SOD activity and POD activity got the maximum,with concentration increasing,the activity declined.Low concentration of copper ion promoted chlorophyll's synthesis,when the copper ion concentration was 20 mg/L,the chlorophyll content of V.radiata seedlings became highest,with the concentration increasing,the chlorophyll content decreased obviously.Conclusion]Copper stress significantly affects the active oxygen scavenging enzyme sys-tem,causes V.radiata seedling's active oxygen metabolism disorder.
Keywords:Copper ion  Vigna radiata  Seedlings  Physiological indicators
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