河川沙塘鳢的ISSR分析河川沙塘鳢的ISSR分析

目的]分析江苏常熟河川沙塘鳢个体大小不一的2个群体之间的遗传差异性。方法]采用ISSR分子标记技术对2个群体进行遗传分析。结果]从77个ISSR引物中筛选出3个引物，对2个群体共24个样品进行扩增，获得27个清晰的扩增位点，其中多态性位点19个，多态位点百分率为70．37％。结果表明，大沙塘鳢的遗传多样性水平略高于小沙塘鳢。大小沙塘鳢群体间的遗传距离为0．2194，群体间的Nei’s遗传分化系数为0．1904。利用MEGA3．0软件构建沙塘鳢24个个体的UPGMA系统树，显示出较明显的分支。结论]江苏常熟河川沙塘鳢种群的遗传多样性水平较低，2个群体的遗传分化已经达到种群分化水平。

ISSR Analysis of Odontobutis potamophila

Objective] The research aimed to study genetic diversity of Odontobutis potamophila in Changshu of Jiangsu from two populations (big and small individuals ).Method] The genetic diversity of two populations was studied using the inter-simple sequence repeat (ISSR) markers.Result]ISSR bands could be differentiated highly in 3 primers after screening.A total of 19 discernible DNA fragments were observed, among which 27 fragments (70.37%) were polymorphic.The percentage of polymorphic (P), Nei′s gene diversity and Shannon′s index showed that the genetic variation of the big population was a little higher than that of the small population.The average genetic distance was 0.219 4 among 24 individuals, and the inter-populations Nei′s gene diversity coefficient were 0.190 4.And UPGMA analysis showed that 24 individuals were divided into two clusters corresponding to two populations.Conclusion] There was low level of genetic diversity of Odontobutis potamophila population in Changshu of Jiangsu.Two groups of genetic differentiation had reached the level of population differentiation.