木霉菌REMI转化子解磷筛选及其解磷效果分析

目的]为了获得具有防病和解磷双重功能的木霉菌REMI转化子。方法]从康氏木霉菌(Trichoderma koningii)T30及其60株REMI(限制性内切酶介导的基因整合技术)转化子中筛选出3株解磷作用较好的转化子,并研究不同碳、氮源对转化子TK!46解磷作用的影响。结果]结果表明,碳源对转化子解磷能力影响较小,而氮源的作用较大,其中转化子在解磷过程中最适氮源是氨态氮。结论]该研究为研制多功能木霉剂奠定了基础,但完全揭示木霉菌解磷机理仍需进一步研究。

Phosphate-dissolving Screening and Effective Analysis of Trichoderma REMI Transformant

Objective The aim of this study was to obtain Trichoderma REMI transformant with double functions of disease prevention and phosphate-dissolving.Method Three preferable phosphate-dissolving transformants were screened out from Trichoderma konigii T30 and its 60 REMI(restriction enzyme mediated DNA integration)transformants.Furthermore,the effect of different carbon and nitrogen sources on phosphate solubilization of TK-46 was investigated.Result Result showed that there was minor influence of carbon sources on the phosphate solubilization.But significant influence of nitrogen sources was found on the phosphate solubilization of Trichoderma.Comparatively NH4 -N was subjected to be utilized more easily by Trichoderma transformant than NO3-N during the solubilization of phosphate.Conclusion This research provided basis for the study of Trichoderma with multiple functions.But there still needed further study to reveal the phosphate-dissolving mechanism of Trichoderma.