复合酶提取灵芝多糖工艺及其抗氧化能力研究

目的]获得复合酶提取灵芝多糖的最佳工艺,探讨灵芝多糖的体外抗氧化能力。方法]以多糖提取率为指标,在单因素试验的基础上,采用正交试验对复合酶用量配比、酶解条件进行优化;采用清除1,1-二苯基苦基苯肼（DPPH）自由基模型评价灵芝多糖的体外抗氧化能力。结果]复合酶提取优于单酶提取,酶用量最佳配比为纤维素酶1.5%、木瓜蛋白酶0.8%、菠萝蛋白酶3.5%（质量分数,相对于底物浓度）;最佳酶解条件为pH5.5,温度50℃、酶解时间为100 min。复合酶提取的灵芝多糖具有良好的清除DPPH自由基作用,其清除能力优于水提灵芝多糖（P〈0.01）,且在浓度0.8～4.8 mg/ml范围内,其对DPPH自由基的清除率随浓度增大而增大。结论]该研究为灵芝多糖的开发提供了科学依据。

Study on Enzymatic Extraction process of Polysaccharide from GANODERMA and Its Oxidation Resistance

Objective] To obtain the optimal enzymatic extraction process of polysaccharide from GANODERMA and discuss its oxidation resistance in vitro.Method]Using extraction rate of polysaccharide as index,the proportion of three enzymes and enzymolysis conditions were optimized by orthogonal test based on single factor test.The in vitro oxidation resistance of polysaccharide was determined by DPPH free radical model.Result] Complex enzyme extraction was superior to single enzyme extraction,and the optimal use levels of complex enzymes were: cellulose of 1.5%,benase of 0.8% and bromelain of 3.5%(mass fraction,relative to concentration of zymolyte).The optimum enzymolysis conditions were as following: pH value of 5.5,temperature of 50 ℃ and enzymolysis duration of 100 min.In comparison with GANODERMA polysaccharide extracted by water,that extracted using complex enzyme was stronger in DPPH radical-scavenging ability(P<0.01),and the DPPH radical-scavenging rate assumed a rise trend along with the increase of polysaccharide concentration in the range of 0.8-4.8 mg/ml.Conclusion] The study provides scientific basis for the development of GANODERMA polysaccharide.