广西猪源沙门菌的分离·鉴定和耐药性研究

目的]了解广西地区猪源沙门菌的流行情况、耐药现状及致病力情况。方法]从广西不同地区11个规模猪场采集99份病料,进行细菌的分离、菌体形态特征观察以及生化鉴定,对分离菌进行常用抗生素药物敏感试验,最后对分离株进行小鼠致病性试验。结果]从99份病料里分离到21株沙门菌,分离率21%。药敏试验表明,分离菌株对阿米卡星、头孢噻肟、氨曲南、左氟沙星具有较高的敏感性,对青霉素G、氨苄西林、阿莫西林、复方新诺明等产生较高的耐药性。致病性试验表明9株分离菌株可导致小白鼠死亡,致死率为43%。结论]分离菌株存在较严重的耐药现象及较强的致病性。该研究为该地区有效控制沙门菌病的发生以及兽医临床上的合理用药提供可靠的理论依据。

Study on the Isolation,Identification and Drug Resistance of Swine Pathogenic Salmonella in Guangxi

Objective ] The research aimed to understand the prevalence, drug resistance and virulence of swine Salmonella in Guangxi. Method ] 99 samples were collected from eleven large-scale pig farms in different regions of Guangxi. Salmonella isolates were identified by staining,morphological features observation and bio-chemical test. All isolates were examined for susceptibility to common antimicrobial agents and virulence tests on mice wee made. Resuh] 21 strains of Salmonella were isolated from 99 samples and the isolation rate was 21%. The drug sensitivity test showed that the isolated strains had higher sensitivity to amikacin, cefotaxime, aztreonam and levofloxacin, while they showed higher resistance to penicillin G, ampicillin, amoxicillin and cotrimoxazole. The virulence test showed that 9 strains of Salmonella could lead to the death of mice and the lethal rate was 43%. Conclusion] The isolated strains had severe resistance to antimicrobials and stronger pathogenicity. The research provided theoretical basis for the effective control of salmonellosis and reasonable application of clinical medicines.