三硝基甲苯对斑马鱼组织ATP酶·乳酸脱氢酶和谷胱甘肽活性的影响

目的]探讨三硝基甲苯(TNT)对斑马鱼(Danio rerio)组织中酶活性的影响,为评价TNT的毒性效应提供参考。方法]采用毒性试验方法研究了不同浓度(0.5、1.0、2.0、4.0 mg/L)的TNT对斑马鱼肝和鳃中ATP酶、头中乳酸脱氢酶(LDH)和肝中谷胱甘肽(GSH)活性的影响。结果]肝和腮组织中的ATP酶活性随浓度的升高而降低;肝中GSH的活性随浓度的升高而升高;头中LDH活性随浓度的升高而降低。酶活随时间的变化规律和随不同浓度的变化规律相同,但变化缓慢。结论]用斑马鱼肝和腮组织中酶活性作为毒理学指标能较好地评价TNT的毒性效应。

Effects of Trinitrotoluene on Activities of ATP Enzyme,Lactate Dehydrogenase and Glutathione from Zebra Fish Tissue

Objective ] The aim was to explore effects of Trinitrotoluene （TNT） on activities of ATP enzyme, lactate dehydmgenase （LDH） and glutathione （GSH） of Danio rerio tissue so as to provide reference for evaluation on toxicity effect of TNT. Method] Effects of TNT of dif- ferent concentrations （0.5, 1.0, 2.0 and 4.0 mg/L） on activities of ATP enzyme from liver and gill tissues, LDH from head tissue and GSH from liver tissue of D. rerio were determined by using toxicity test method. Result] The activity of ATP enzyme from liver and gill tissues de- creased with the increase of TNT mass concentration; the activity of GSH from liver tissue increased with the increase of TNT mass concentra- tion ; the activity of LDH from head tissue decreased with the increase of TNT mass concentration. The variation of enzyme activity over time was the same as different concentration but changed slowly. Conclusion] It can better evaluate the toxic effects of TNT to use the activities of enzymes from D. rerio liver and gills as toxicological indicators.