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海南野生蕉组培苗栽培基质配方筛选
引用本文:陈友,光虹宇,冯慧敏,冯素萍,武耀廷.海南野生蕉组培苗栽培基质配方筛选[J].安徽农业科学,2013(2):519-520,523.
作者姓名:陈友  光虹宇  冯慧敏  冯素萍  武耀廷
作者单位:琼州学院,海南三亚,572022;琼州学院,海南三亚,572022;琼州学院,海南三亚,572022;琼州学院,海南三亚,572022;琼州学院,海南三亚,572022
基金项目:农业部热带种质资源保护项目(12RZZY-47);琼州学院青年教师科研基金项目(No.QY200917);三亚市院校专项资金项目(No.YD09041)
摘    要:目的]筛选适合海南野生蕉组培苗培养的栽培基质。方法]以海南野生蕉吸芽茎尖组培苗为材料,以普通红泥土壤为对照,分别采用椰糠、木屑、珍珠岩、河沙配成14组不同的基质,测定各基质中香蕉组培苗的叶片数量变化、新叶展开宽度变化、假茎高度变化和粗度变化4个生长指标。结果]最适合海南野生蕉吸芽茎尖组培苗生长的基质为木屑∶河沙=1∶1(V/V)的基质,其次为木屑∶珍珠岩=1∶2(V/V)的基质及椰糠∶珍珠岩比例=1∶2(V/V)的基质。结论]该研究为香蕉野生种质资源的保护提供了科学基础。

关 键 词:海南野生蕉  组培苗  基质  筛选

Formula Screening of Cultivation Matrix for Tissue Culture Seedlings of Hainan Wild Banana
Institution:CHEN You et al(QiongZhou University,Sanya,Hainan 572022)
Abstract:Objective] The study was to screen the cultivation matrix for tissue culture seedlings of Hainan wild banana.Method] With suckers and shoot tip tissue culture seedlings of Hainan wild banana as materials and the ordinary red soil as CK,14 different matrices were prepared with coconut husk,sawdust,perlite,and river sand,and leaf number changes,of new leaf unfolding width changes,height variation and crude changes of pseudostem 4 growth indexes of banana tissue culture seedlings in each matrix were detected.Result] The optimal matrix for the growth of suckers stem tip tissue culture seedlings of Hainan wild banana was sawdust∶river sand = 1∶1(V/V) matrix,Secondly,it were sawdust∶perlite=1∶2(V/V) matrix and coconut husk∶perlite=1∶2(V/V) matrix.Conclusion] The study provided a scientific basis for the germplasm resource protection of wild banana.
Keywords:Hainan wild banana  Tissue culture seedling  Cultivation matrix  Screening
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