5种蔷薇遗传多态性的AFLP分析

目的]研究AFLP标记在蔷薇遗传多态性方面的应用和5种不同蔷薇品种的遗传背景。方法]应用10条人工设计的与接头序列相识别的AFLP选择性引物,用PstⅠ酶切,对5种蔷薇基因组DNA进行AFLP反应,分析不同蔷薇间的遗传相似系数和遗传距离。结果]获得了108个AFLP标记,单引物获得的标记数在4～16,5种蔷薇的遗传相似系数为0.824(0.732～0.947),遗传距离为0.053～0.268。结论]该研究为评价蔷薇的遗传稳定性提供了相关的参数。

AFLP Analysis of Genetic Polymorphism of Five Lines Rose

Objective] The research aimed to study the application of AFLP markers in detecting genetic polymorphic loci in 5 strains rose and their genetic background.Method] Ten human-designed AFLP selective primers different from adaptor sequence were used,genomic DNA from 5 strains rose were restrictive by PstⅠ.And their Genetic similarity coefficient and genetic distance were studied.Result] The 108 AFLP markers were obtained,and the obtaining marker numbers of individual primer were 4-16.The similarity index of population in five strains rose was 0.824(0.732-0.947),and the genetic distance were 0.053-0.268.Conclusion] AFLP technique was an effective method in genetic polymorphism monitoring of rose.